Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology
| Autor: | Miwaka Ohtani, Taro Saito, Dai Hirata, Ayaka Saka, Satoru Nogami, Masashi Yukawa, Fumi Sano-Kumagai, Hiroshi Sawai, Shinichi Morishita, Genjiro Suzuki, Jun Sese, Yoshikazu Ohya |
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| Rok vydání: | 2005 |
| Předmět: |
Cell division
biology Fission Saccharomyces cerevisiae Image processing General Medicine biology.organism_classification Bioinformatics Cell morphology Yeast Digital image Microscopy Fluorescence Schizosaccharomyces pombe Genetics Image Processing Computer-Assisted Biological system Cell Division Software |
| Zdroj: | Current genetics. 49(4) |
| ISSN: | 0172-8083 |
| Popis: | To study the cellular functions of gene products, various yeast morphological mutants have been investigated. To describe yeast morphology objectively, we have developed image processing programs for budding and fission yeast. The programs, named CalMorph for budding yeast and F-CalMorph for fission yeast, directly process microscopic images and generate quantitative data about yeast cell shape, nuclear shape and location, and actin distribution. Using CalMorph, we can easily and quickly obtain various quantitative data reproducibly. To study the utility and reliability of CalMorph, we evaluated its data in three ways: (1) The programs extracted three-dimensional bud information from two-dimensional digital images with a low error rate ( |
| Databáze: | OpenAIRE |
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