Linoleic and oleic acids enhance cell migration by altering the dynamics of microtubules and the remodeling of the actin cytoskeleton at the leading edge
| Autor: | Cristian Gabriel Acosta, Mariano Bisbal, Patricia Kunda, N Lujea, M Masner |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
rac1 GTP-Binding Protein
0301 basic medicine Cell biology Science Cell Cell leading edge Microtubules Time-Lapse Imaging Article Linoleic Acid 03 medical and health sciences 0302 clinical medicine Single-cell analysis Cell Movement Cell Line Tumor medicine Ascitic Fluid Humans Cytoskeleton Cancer Ovarian Neoplasms Multidisciplinary Dose-Response Relationship Drug Chemistry Cell migration Actin cytoskeleton Up-Regulation Gene Expression Regulation Neoplastic Actin Cytoskeleton 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Medicine Female Single-Cell Analysis Lamellipodium Filopodia Oleic Acid |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-94399-8 |
| Popis: | Fatty acids (FA) have a multitude of biological actions on living cells. A target of their action is cell motility, a process of critical importance during cancer cell dissemination. Here, we studied the effect of unsaturated FA on ovarian cancer cell migration in vitro and its role in regulating cytoskeleton structures that are essential for cell motility. Scratch wound assays on human ovary cancer SKOV-3 cell monolayers revealed that low doses (16 μM) of linoleic acid (LA, 18:2 ω6) and oleic acid (OA; 18:1 ω9) promoted migration, while α-linolenic acid (ALA, 18:3 ω3), showed a migration rate similar to that of the control group. Single cell tracking demonstrated that LA and OA-treated cells migrated faster and were more orientated towards the wound closure than control. In vitro addition of those FA resulted in an increased number, length and protrusion speed of filopodia and also in a prominent and dynamic lamellipodia at the cell leading edge. Using time-lapse video-microscopy and FRAP we observed an increase in both the speed and frequency of actin waves associated with more mobile actin and augmented Rac1 activity. We also observed that FA induced microtubule-organizing center (MTOC)-orientation towards the cell front and affected the dynamics of microtubules (MT) in the direction of cell migration. We propose that environmental cues such as OA and LA present in ascitic fluid, should be taken into account as key factors for the regulation of cell migration. |
| Databáze: | OpenAIRE |
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