Construction of engineeredSaccharomyces cerevisiaestrain to improve that whole-cell biocatalytic production of melibiose from raffinose
| Autor: | Yuanxia Sun, Yingbiao Zhou, Caixia Dong, Yan Men, Juankun Zhang, Yueming Zhu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins 030106 microbiology Saccharomyces cerevisiae Bioengineering Fructose Protein Engineering Applied Microbiology and Biotechnology Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound Raffinose Melibiose Gene biology Galactose Fructose transport biology.organism_classification Yeast chemistry Biochemistry alpha-Galactosidase Biocatalysis Microorganisms Genetically-Modified Gene Deletion Biotechnology |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 44:489-501 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-017-1901-8 |
| Popis: | There are excessive by-products in the biocatalysis process of this whole-cell biocatalytic production of melibiose from raffinose with current Saccharomyces cerevisiae strains. To solve this problem, we constructed engineered strains based on a liquor yeast (S. cerevisiae) via gene deletion (mel1 gene), heterologous integration (fsy1 or/and ffzi1 gene from Candida magnoliae), and gene overexpression (gcr1 gene). Functional verification showed that deletion of the mel1 gene led to elimination of the reactions catalyzed by α-galactosidase, as well as elimination of the degradation of melibiose and the formation of galactose by-product. Insertion of the fsy1 or/and ffzi1 gene and overexpression of the gcr1 gene could contribute to fructose transport for enhancing the biopurification rate of the fructose by-product. Compared with the wild-type strain, the optimal engineered strain of MP8 (Δmel1::fsy1 cm::ffzi1 cm::gcr1 sc) had improved about 30% on yield, 31% on productivity, and 36% on purity of the melibiose product. |
| Databáze: | OpenAIRE |
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