Single intratracheal administration of cross-linked water-soluble acrylic acid polymer causes acute alveolo-interstitial inflammation and the subsequent fibrotic formation possibly via the TGF-β1 pathway in the lung of rats
| Autor: | Masayuki Yokoyama, Takamasa Kido, Takashi Okano, Eri Hachisuka, Hiroshi Hano, Machi Suka, Hiroto Okoshi, Takashi Yamauchi, Hiroyuki Yanagisawa, Wataru Yoshioka |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Polymers Pulmonary Fibrosis Inflammation Toxicology Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine Fibrosis Pulmonary fibrosis medicine Animals Lung biology medicine.diagnostic_test Chemistry Pneumoconiosis medicine.disease Rats Inbred F344 Rats Fibronectin Pulmonary Alveoli Trachea 030104 developmental biology medicine.anatomical_structure Bronchoalveolar lavage Cross-Linking Reagents Acrylates Myeloperoxidase biology.protein medicine.symptom 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Toxicology. 448 |
| ISSN: | 1879-3185 |
| Popis: | In a Japanese chemical factory, a lung disease like pneumoconiosis appeared at a high rate among workers handling cross-linked water-soluble acrylic acid polymer (CWAAP). To our knowledge, no such case was known in the world until very recently. The present study was designed to elucidate the effect of single intratracheal CWAAP instillation on the lung of rats. The CWAAP group had a significant increase in relative lung weight accompanied by a significant elevation in the number of total cells, total protein concentrations, and myeloperoxidase concentrations in bronchoalveolar lavage fluid when compared to the control group. The histopathological study revealed acute lung inflammation with the destruction of alveoli. The factors promoting fibrosis, macrophages, TGF-β1, collagen and fibronectin vs. the factors suppressing fibrosis, matrix metalloproteinases were more powerfully driven in the CWAAP group, resultantly leading to fibrotic formation. In turn, we examined if acute lung inflammation and the subsequent fibrotic formation seen in the CWAAP group appeared in the other water-soluble polymer groups. Their histopathological findings were observed only in the polyacrylic acid sodium (PAAS), a monomer of CWAAP, group. The degree of inflammation and fibrogenesis was stronger in the CWAAP group than in the PAAS group. In conclusion, the present study demonstrated the induction of acute lung inflammation and the subsequent fibrotic formation by single intratracheal CWAAP instillation. The structural features of CWAAP that contains many carboxyl groups and cross-linked chains may be responsible for enhanced inflammation and fibrogenesis in the lung. |
| Databáze: | OpenAIRE |
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