Unraveling Human AQP5-PIP Molecular Interaction and Effect on AQP5 Salivary Glands Localization in SS Patients
| Autor: | Yvonne Myal, Florent Lhotellerie, Clara Chivasso, Christine Delporte, Benoit Vanhollebeke, Veronika Nesverova, Kevin L. Schey, Stefania Moscato, François Chaumont, Muhammad Shahnawaz Soyfoo, Chiara Baldini, Karelle Leroy, Zhen Wang, Jason Perret, Susanna Törnroth-Horsefield, Bruna Rayane Teodoro Junqueira, Michael Järvå, Letizia Mattii, Egor Zindy, Valérie Delforge, Anne Blanchard, Kristie L. Rose, Fredrik Öberg, Maud Martin, Nargis Bolaky |
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| Jazyk: | francouzština |
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
QH301-705.5 Knockout aquaporin-5 Saliva secretion Aquaporin Chromosomal translocation salivary gland Acinar Cells Article Salivary Glands Cell Line 03 medical and health sciences Mice 0302 clinical medicine stomatognathic system parasitic diseases medicine Animals Humans prolactin-inducible protein Biology (General) 030304 developmental biology Mice Knockout 0303 health sciences Binding Sites Salivary gland Chemistry Aquaporin-5 Prolactin-inducible protein Sjögren’s syndrome Aquaporin 5 Membrane Transport Proteins Protein Binding Sjogren's Syndrome fungi General Medicine Apical membrane Sciences bio-médicales et agricoles In vitro Cell biology medicine.anatomical_structure Prolactin-Inducible Protein 030220 oncology & carcinogenesis Knockout mouse lipids (amino acids peptides and proteins) |
| Zdroj: | Cells, 10 Cells Volume 10 Issue 8 Cells, Vol 10, Iss 2108, p 2108 (2021) |
| Popis: | Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sjögren’s syndrome (SS) display abnormal AQP5 localization within acinar cells from SGs that correlate with sicca manifestation and glands hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate AQP5 trafficking as observed in lacrimal glands from mice. However, the role of the AQP5-PIP complex remains poorly understood. In the present study, we show that PIP interacts with AQP5 in vitro and in mice as well as in human SGs and that PIP misexpression correlates with an altered AQP5 distribution at the acinar apical membrane in PIP knockout mice and SS hMSG. Furthermore, our data show that the protein-protein interaction involves the AQP5 C-terminus and the N-terminal of PIP (one molecule of PIP per AQP5 tetramer). In conclusion, our findings highlight for the first time the role of PIP as a protein controlling AQP5 localization in human salivary glands but extend beyond due to the PIP-AQP5 interaction described in lung and breast cancers. |
| Databáze: | OpenAIRE |
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