Automation of antimicrobial activity screening
| Autor: | Daneli Lopez-Perez, Meggan C. Madonna, Samuel P. Forry, Madeleine D. Pasco, Nancy J. Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient Minimum bactericidal concentration Antimicrobial activity screening 030106 microbiology Biophysics Biofilm Cetylpyridinium Biology Antimicrobial biology.organism_classification Cetylpyridinium chloride Applied Microbiology and Biotechnology Streptococcus mutans Microbiology 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Automation Performance assessment 030104 developmental biology food chemistry Agar Original Article |
| Zdroj: | AMB Express |
| ISSN: | 2191-0855 |
| Popis: | Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with tooth decay. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50 % inhibitory concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect of the counter ion on antimicrobial activity. Electronic supplementary material The online version of this article (doi:10.1186/s13568-016-0191-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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