Further studies on the relationship between platelet buoyant density and platelet age
| Autor: | Pierre Sié, C. Caranobe, F. Vigoni, B. Boneu, A. Boneu |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Blood Platelets
Differential centrifugation medicine.medical_specialty education.field_of_study Time Factors Isotopes of chromium Cell Survival Chemistry Population Platelet life span Buoyant density Hematology Centrifugation Isopycnic Chromium Radioisotopes Calculation methods Platelet survival study Kinetics Endocrinology Internal medicine Immunology medicine Humans Platelet education |
| Zdroj: | American Journal of Hematology. 13:239-246 |
| ISSN: | 1096-8652 0361-8609 |
| DOI: | 10.1002/ajh.2830130307 |
| Popis: | The relationship between platelet buoyant density and platelet age was investigated in eight human subjects submitted to an autologous chromium labeled platelet survival study. Platelets were isolated after isopycnic centrifugation using eight discontinuous isoosmotic stractan gradients (five subjects), or various continuous and linear isoosmolar gradients (three subjects). A paradoxical radioactivity enrichment of the dense platelets and a premature loss of radioactivity in the light platelets were observed. These results are explained by a shift of the radioactivity distribution curve toward higher densities during the 3-4 days after platelet injection, while the standard deviation of the distribution was conserved throughout the platelet life span. These results suggest that young platelets are heterogeneous and slightly less dense than the total platelet population. |
| Databáze: | OpenAIRE |
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