Somatic Hypermutations in the Immunoglobulin Genes of Two New Human Lymphoma Lines of Lymphatic Follicle Origin
| Autor: | T. Tuomikoski, Hongyan Wu, Mine Eray, Sakari Knuutila, Matti Kaartinen, Petri S. Mattila |
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| Rok vydání: | 1994 |
| Předmět: |
Somatic cell
Molecular Sequence Data Immunology Follicular lymphoma Translocation Genetic Homology (biology) Germline 03 medical and health sciences 0302 clinical medicine Germline mutation Tumor Cells Cultured medicine Humans RNA Messenger Lymphoma Follicular Gene 030304 developmental biology Chromosomes Human Pair 14 Genetics 0303 health sciences Base Sequence Genes Immunoglobulin biology General Medicine medicine.disease Molecular biology 3. Good health Lymphoma Immunoglobulin J-Chains 030220 oncology & carcinogenesis Mutation biology.protein Antibody Chromosomes Human Pair 18 Immunoglobulin Heavy Chains |
| Zdroj: | Scandinavian Journal of Immunology. 39:295-300 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91 % homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes. |
| Databáze: | OpenAIRE |
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