Evolution of hepatitis G virus infection and antibody response to envelope protein in patients with transfusion‐associated non‐A, non‐B hepatitis
| Autor: | K. Shimoda, Michael Tacke, Eiji Tanaka, Kunihiko Hino, S. Schmolke, Alfred Engel, Georg Hess, Kendo Kiyosawa |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Hepatitis Viral Human Hepatitis C virus Hepacivirus medicine.disease_cause Virus Viral Envelope Proteins Virology Prevalence Humans Medicine Clinical significance Hepatitis Antibodies Antigens Viral Hepatitis Hepatology biology business.industry Flaviviridae Transfusion Reaction virus diseases RNA Hepatitis C Antibodies Middle Aged medicine.disease Hepatitis C digestive system diseases Reverse transcriptase Infectious Diseases Acute Disease biology.protein RNA Viral Female Antibody business Nested polymerase chain reaction Follow-Up Studies |
| Zdroj: | Journal of Viral Hepatitis. 5:153-159 |
| ISSN: | 1365-2893 1352-0504 |
| DOI: | 10.1046/j.1365-2893.1998.00095.x |
| Popis: | The clinical significance and course of acute hepatitis G virus (HGV) infection were studied by measuring HGV RNA and antibody to HGV envelope protein E2 (HGV-E2 antibody). A total of 59 patients with transfusion-associated non-A, non-B hepatitis, who were followed-up for more than 1 year, were selected retrospectively. HGV RNA was measured by reverse transcriptase (RT) and nested polymerase chain reaction (PCR) was performed, using primer sets, in the 5'-non-coding region of the HGV genome. HGV-E2 antibody was measured by enzyme-linked immunosorbent assay (ELISA) using recombinant E2 protein. Of the 59 patients, 51 (86%) were infected with hepatitis C virus (HCV) and 12 (20%) were infected with HGV; 11 of the 12 with HGV infection were also infected with HCV. HGV viraemia was cleared during the follow-up period in seven of the 12 patients with HGV infection. All these seven patients seroconverted for HGV-E2 antibody just before or just after the clearance of HGV viraemia. In contrast, all five patients without clearance of HGV viraemia were negative for HGV-E2 antibody (P = 0.0013). Of seven patients with continuous HGV viraemia at 1 year from the onset of acute hepatitis, four with HCV RNA showed chronic elevation of alanine aminotransferase (ALT) but three without HCV RNA did not. The severity of acute hepatitis was similar between patients with both HGV and HCV infections and in those with HCV infection alone. The majority of patients with HGV infection cleared the virus during long-term follow-up. Appearance of HGV-E2 antibody was associated with the clearance of HGV viraemia. An abnormal ALT level was noted to depend on HCV infection but not on HGV infection in both the acute and chronic phases of transfusion-associated hepatitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|