Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte-myocardial cell co-culture system

Autor: Haiying Li, Shuang Lin, Xiaochun Weng, Anwu Huang, Zehao Jin, Ye Chen
Rok vydání: 2020
Předmět:
0301 basic medicine
Medicine (General)
Cyclic AMP Responsive Element Binding Protein
Myocardial Ischemia
Acute phase response
030204 cardiovascular system & hematology
Biochemistry
Rats
Sprague-Dawley
0302 clinical medicine
co-culture system
Medicine
Myocytes
Cardiac
RNA
Small Interfering
Cyclic AMP Response Element-Binding Protein
Cells
Cultured
Heat-Shock Proteins
Acute-phase protein
General Medicine
Endoplasmic Reticulum Stress
humanities
Cell Hypoxia
Cell biology
medicine.anatomical_structure
Liver
Hepatocyte
Gene Knockdown Techniques
Myocardial hypoxia
hypoxia/reoxygenation
Signal Transduction
myocardial hypoxia–reperfusion injury
inflammatory cytokine
Cell Survival
cyclic AMP-responsive element-binding protein H
Primary Cell Culture
Cleavage (embryo)
Proinflammatory cytokine
Pre-Clinical Research Report
03 medical and health sciences
R5-920
Animals
Humans
Acute-Phase Reaction
business.industry
Interleukin-6
Tumor Necrosis Factor-alpha
Myocardium
Biochemistry (medical)
Cell Biology
medicine.disease
Coculture Techniques
Rats
Disease Models
Animal
030104 developmental biology
Animals
Newborn
Myocardial cell
Hepatocytes
business
Reperfusion injury
Zdroj: The Journal of International Medical Research
Journal of International Medical Research, Vol 48 (2020)
ISSN: 1473-2300
Popis: Objective This study aimed to determine whether proinflammatory cytokines have an effect on myocardial cells (MCs) and hepatocytes during myocardial ischemia to induce cyclic AMP-responsive element-binding protein H (CREBH) cleavage, activate the acute phase response in the liver, and cause a superimposed injury in MCs. Methods In this study, a hepatocyte–MC transwell co-culture system was used to investigate the relationship between myocardial hypoxia/reperfusion injury and CREBH cleavage. MCs and hepatocytes of neonatal rats were obtained from the ventricles and livers of Sprague–Dawley rats, respectively. MCs were inoculated into the lower chamber of transwell chambers for 12 hours under hypoxia. Levels of the endoplasmic reticulum stress protein glucose-regulated protein 78 in MCs, CREBH in hepatocytes, inflammatory factor (tumor necrosis factor-α and interleukin-6) levels, and cell viability were evaluated. The effect of CREBH knockdown was also studied using a CREBH-specific short hairpin RNA (Ad-CREBHi). Results We found that proinflammatory cytokines affect MCs and hepatocytes during myocardial ischemia to induce CREBH cleavage, activate the acute phase response in the liver, and cause superimposed injury in MCs. Conclusions Expression of CREBH aggravates myocardial injury during myocardial ischemia.
Databáze: OpenAIRE
Externí odkaz: