Intestinal Immune System Modulating Polysaccharides from Rhizomes ofAtractylodes lancea
| ISSN: | 1439-0221 0032-0943 |
|---|---|
| DOI: | 10.1055/s-2006-957564 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9bf8019b46d292e11c5486165a5ea7f https://doi.org/10.1055/s-2006-957564 |
| Přírůstkové číslo: | edsair.doi.dedup.....a9bf8019b46d292e11c5486165a5ea7f |
| Autor: | Hiroaki Kiyohara, Haruki Yamada, Kwang-Won Yu, Tsukasa Matsumoto, Han-Chul Yang |
| Rok vydání: | 1998 |
| Předmět: |
Pharmaceutical Science
Pronase Uronic acid Polysaccharide Analytical Chemistry Peyer's Patches chemistry.chemical_compound Adjuvants Immunologic Polysaccharides Drug Discovery Cells Cultured Pharmacology chemistry.chemical_classification Plants Medicinal biology Molecular mass Organic Chemistry Biological activity Chromatography Ion Exchange biology.organism_classification Complementary and alternative medicine Biochemistry chemistry Atractylodes Molecular Medicine Atractylodes lancea ARAF |
| Zdroj: | Planta Medica. 64:714-719 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-2006-957564 |
| Popis: | Hot water extract (ALR-0) of rhizomes of Atractylodes lanceo DC. was fractionated into MeOH-soluble fraction (ALR-1), supernatant fraction of EtOH precipitation (ALR-3 + 4), and crude polysaccharide fraction (ALR-5). Among these fractions, only ALR-5 showed potent stimulating activity for proliferation of bone marrow cells mediated by Peyer's patch cells. ALR-5 gave three potently active carbohydrate-rich fractions (ALR-5IIa, 5IIb, and 5IIc) by anion-exchange chromatography on DEAE-Sepharose CL-6B, and three active polysaccharides (ALR-5IIa-1-1, ALR-5IIb-2-2, and ALR-5IIc-3-1) were further purified from the respective fractions. The order of activity was revealed to be ALR-5IIb-2-2 > or = ALR-5IIa-1-1 > ALR-5IIc-3-1, ALR-5IIa-1-1, 5IIb-2-2, and 5IIc-3-1 each was eluted as a single peak on HPLC and their molecular weights were estimated to be 74,000, 3,100, 16,000, respectively. ALR-5IIa-1-1 consisted mainly of Ara and Gal (molar ratio; 0.6: 1.0) in addition to a trace amount of uronic acid whereas ALR-5IIb-2-2 and ALR-5IIc-3-1 mainly comprised Ara, Gal, GlcA, and GalA (molar ratio; 0.2: 1.0: 0.2: 0.8, and 0.5: 1.0: 0.7: 1.5, respectively). Methylation analysis indicated that ALR-5IIa-1-1 consisted mainly of terminal Araf, 4- or 5-linked Ara, 3.4- or 3.5-branched Ara, and 3-linked, 4-linked, and 3,6-branched Gal. ALR-5IIb-2-2 and ALR-5IIc-3-1 were composed mainly of terminal Araf, 4- or 5-linked Ara, 4-linked Gal, 4-linked GalA, and terminal GlcA. In addition, ALR-5IIb-2-2 mainly comprised 4-linked Xyl whereas ALR-5IIc-3-1 consisted mainly of 2,4-branched Rha. Single radial gel diffusion indicated that ALR-5IIa-1-1 showed a strong reactivity with beta-glucosyl-Yariv antigen, whereas ALR-5IIb-2-2 and ALR-5IIc-3-1 did not show the reactivity with the antigen. Treatments of ALR-5IIa with NalO4, NaClO2 and pronase did not reduce the stimulating activity for Peyer's patch cells, however combination of exo-alpha-L-arabinofuranosidase and exo-beta-D-(1-->3)-galactanase digestions of ALR-5IIa-1-1 significantly decreased its activity. |
| Databáze: | OpenAIRE |
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