Industrial kinetic resolution of d ,l -pantolactone by an immobilized whole-cell biocatalyst
| Autor: | Liu-Nv Huang, Wen-Fang Luo, Yi-Bin Tang, Qiu-Hua Zhang, Liu Yang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Calcium alginate
biology Chemistry General Chemical Engineering General Chemistry equipment and supplies biology.organism_classification Combinatorial chemistry Catalysis law.invention Kinetic resolution Pichia pastoris Hydrolysis chemistry.chemical_compound Stereospecificity Biocatalysis law Recombinant DNA |
| Zdroj: | RSC Advances. 11:30373-30376 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/d1ra05708a |
| Popis: | Immobilized whole-cells of Pichia pastoris harboring recombinant D-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of D,L-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of D-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity. |
| Databáze: | OpenAIRE |
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