A multiplexed three-dimensional paper-based electrochemical impedance device for simultaneous label-free affinity sensing of total and glycated haemoglobin: The potential of using a specific single-frequency value for analysis
| Autor: | Orawon Chailapakul, Wanida Laiwattanapaisal, Yuwadee Boonyasit |
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| Rok vydání: | 2016 |
| Předmět: |
Paper
Time Factors Value (computer science) 02 engineering and technology Electrochemistry 01 natural sciences Biochemistry Analytical Chemistry Limit of Detection Diabetes Mellitus Electric Impedance Humans Environmental Chemistry Electrodes Electrical impedance Glycated haemoglobin Spectroscopy Glycated Hemoglobin Detection limit Reproducibility Chromatography Chemistry 010401 analytical chemistry Equipment Design 021001 nanoscience & nanotechnology Boronic Acids 0104 chemical sciences Electrode Eggshell membrane 0210 nano-technology Biomarkers Blood Chemical Analysis |
| Zdroj: | Analytica Chimica Acta. 936:1-11 |
| ISSN: | 0003-2670 |
| Popis: | A novel three-dimensional paper-based electrochemical impedance device (3D-PEID) is first introduced for measuring multiple diabetes markers. Herein, a simple 3D-PEID composed of a dual screen-printed electrode on wax-patterned paper coupled with a multilayer of magnetic paper was fabricated for label-free electrochemical detection. The results clearly demonstrated in a step-wise manner that the haptoglobin (Hp)-modified and 3-aminophenylboronic acid (APBA)-modified eggshell membranes (ESMs) were highly responsive to a clinically relevant range of total (0.5-20 g dL(-1); r(2) = 0.989) and glycated haemoglobin (HbA1c) (2.3%-14%; r(2) = 0.997) levels with detection limits (S/N = 3) of 0.08 g dL(-1) and 0.21%, respectively. The optimal binding frequencies of total haemoglobin and HbA1c to their specific recognition elements were 5.18 Hz and 9.99 Hz, respectively. The within-run coefficients of variation (CV) were 1.84%, 2.18%, 1.72%, and 2.01%, whereas the run-to-run CVs were 2.11%, 2.41%, 2.08%, and 2.21%, when assaying two levels of haemoglobin and HbA1c, respectively. The CVs for the haemoglobin and HbA1c levels measured on ten independently fabricated paper-based sheets were 1.96% and 2.10%, respectively. These results demonstrated that our proposed system achieved excellent precision for the simultaneous detection of total haemoglobin and HbA1c, with an acceptable reproducibility of fabrication. The long-term stability of the Hp-modified eggshell membrane (ESM) was 98.84% over a shelf-life of 4 weeks, enabling the possibility of storage or long-distance transport to remote regions, particularly in resource-limited settings; however, for the APBA-modified ESM, the stability was 92.35% over a one-week period. Compared with the commercial automated method, the results demonstrated excellent agreement between the techniques (p-value 0.05), thus permitting the potential application of 3D-PEID for the monitoring of the glycaemic status in diabetic patients. |
| Databáze: | OpenAIRE |
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