Double trans-chromosomic mice: Maintenance of two individual human chromosome fragments containing Ig heavy and κ loci and expression of fully human antibodies
| Autor: | Tokuyuki Shinohara, Hiroshi Uejima, Isao Ishida, Atsuko Ohguma, Kazuma Tomizuka, Kaoru Sato, Mitsuo Oshimura, Sonoko Tanaka, Hitoshi Yoshida |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Somatic cell medicine.drug_class Enzyme-Linked Immunosorbent Assay Mice Transgenic Locus (genetics) Hybrid Cells Immunoglobulin light chain Monoclonal antibody Antibodies Immunoglobulin kappa-Chains Mice medicine Animals Chromosomes Human Humans Crosses Genetic In Situ Hybridization Fluorescence Serum Albumin Mice Knockout Multidisciplinary biology Antibodies Monoclonal Chromosome Fibroblasts Biological Sciences Molecular biology Microcell-mediated chromosome transfer biology.protein Female Human genome Antibody Immunoglobulin Heavy Chains |
| Zdroj: | Proceedings of the National Academy of Sciences. 97:722-727 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The use of a human chromosome or its fragment as a vector for animal transgenesis may facilitate functional studies of large human genomic regions. We describe here the generation and analysis of double trans-chromosomic (Tc) mice harboring two individual human chromosome fragments (hCFs). Two transmittable hCFs, one containing the Ig heavy chain locus ( IgH , ≈1.5 Mb) and the other the κ light chain locus ( Ig κ , ≈2 Mb), were introduced into a mouse strain whose endogenous IgH and Ig κ loci were inactivated. In the resultant double-Tc/double-knockout mice, substantial proportion of the somatic cells retained both hCFs, and the rescue in the defect of Ig production was shown by high level expression of human Ig heavy and κ chains in the absence of mouse heavy and κ chains. In addition, serum expression profiles of four human Ig γ subclasses resembled those seen in humans. They mounted an antigen-specific human antibody response upon immunization with human serum albumin, and human serum albumin-specific human monoclonal antibodies with various isotypes were obtained from them. These results represent a generation of mice with “humanized” loci by using the transmittable hCFs, which suggest that the Tc technology may allow for the humanization of over megabase-sized, complex loci in mice or other animals. Such animals may be useful not only for studying in vivo functions of the human genome but also for obtaining various therapeutic products. |
| Databáze: | OpenAIRE |
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