Synergy between Polyethylenimine and Different Families of Antibiotics against a Resistant Clinical Isolate of Pseudomonas aeruginosa
| Autor: | Ru-tao Wang, Zhao Wang, Renée Riffon, Hayssam Khalil, Tao Chen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ofloxacin
Cefotaxime medicine.drug_class Antibiotics Ceftazidime macromolecular substances Microbial Sensitivity Tests Biology Pharmacology Microbiology Ciprofloxacin Drug Resistance Bacterial medicine Humans Polyethyleneimine Ticarcillin Pseudomonas Infections Pharmacology (medical) Piperacillin technology industry and agriculture Drug Synergism biochemical phenomena metabolism and nutrition Carbenicillin Antimicrobial Anti-Bacterial Agents Aminoglycosides Infectious Diseases Susceptibility Pseudomonas aeruginosa Tobramycin Rifampin Novobiocin medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 52:1635-1641 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01071-07 |
| Popis: | The in vitro activities of 10 families of antimicrobial agents alone and in combination with a synthetic polycationic polymer, polyethylenimine (PEI), against a resistant clinical isolate of Pseudomonas aeruginosa were investigated by MIC assays, checkerboard testing, and killing curve studies. At a concentration of 250 nM, PEI (10 kDa) was not directly bactericidal or bacteriostatic; but when it was used in combination with novobiocin, ceftazidime, ampicillin, ticarcillin, carbenicillin, piperacillin, cefotaxime, chloramphenicol, rifampin, or norfloxacin, it significantly reduced the MICs of these antibiotics by 1.5- to 56-fold. However, the MICs of aminoglycosides, polymyxins, and vancomycins were increased by 1.2- to 5-fold when these drugs were combined with PEI; and the MICs of tetracycline, erythromycin, ciprofloxacin, and ofloxacin were not affected when these drugs were combined with PEI. In the killing curve studies, combinations of PEI with novobiocin, ceftazidime, chloramphenicol, or rifampin resulted in 5- to 8-log 10 CFU/ml reductions in bacterial counts when 25% of the MIC of each antibiotic was used. These results indicate that infections due to resistant Pseudomonas strains could be treated by the use of a synergistic combination of PEI and antimicrobial drugs. |
| Databáze: | OpenAIRE |
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