Developmental Changes in mRNA Encoding Cardiac Na+/H+ Exchanger (NHE-1) in Rabbit
| Autor: | F.Y. Chen, C. Vandop, Jay M. Jarmakani |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Cellular activity Sodium-Hydrogen Exchangers Heart Ventricles Molecular Sequence Data Biophysics Biology Polymerase Chain Reaction Biochemistry Contractility medicine Animals RNA Messenger Molecular Biology DNA Primers Acidosis Fetus Messenger RNA Base Sequence Myocardium Gene Expression Regulation Developmental Nuclease protection assay Cell Biology Molecular biology Sodium–hydrogen antiporter Animals Newborn Gestation Rabbits medicine.symptom |
| Zdroj: | Biochemical and Biophysical Research Communications. 212:960-967 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.2063 |
| Popis: | The faster recovery of cardiac contractility in newborn rabbit hearts during acute acidosis compared to adult hearts correlates with greater cellular activity of the Na + /H + exchanger. We quantified mRNA encoding Na + /H + exchanger-1 (NHE-1) in rabbit ventricles of fetal (27 days gestation), newborn (2-5 days), and adult (>6 months) New Zealand white rabbits using reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay, with GAPD mRNA as standard. Both RT-PCR and RNase protection assay revealed similar (p>0.05) cardiac levels of NHE-1 mRNA in fetal and newborn hearts, while NHE-1 mRNA in these hearts was 1.7x and 1.6x greater than in adult hearts by both RT-PCR and RNase protection assay. These developmental changes in NHE-1 mRNA correlate with the developmental changes in Na + /H + activity in sarcolemmal vesicles purified from rabbit heart. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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