Contractile Response of Bovine Lateral Saphenous Vein to Ergotamine Tartrate Exposed to Different Concentrations of Molecularly Imprinted Polymer
| Autor: | Manoj B. Kudupoje, Eric S. Vanzant, Kyle R. McLeod, Alexandros Yiannikouris, James L. Klotz, Karl A. Dawson |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Molar concentration Health Toxicology and Mutagenesis lcsh:Medicine In Vitro Techniques Toxicology ergot alkaloids Article Molecular Imprinting 03 medical and health sciences myograph imprinted polymer medicine Ergotamine Animals Vasoconstrictor Agents Saphenous Vein IC50 EC50 Chromatography Chemistry lcsh:R 0402 animal and dairy science Molecularly imprinted polymer 04 agricultural and veterinary sciences 040201 dairy & animal science Bioavailability 030104 developmental biology Ergotamine Tartrate NIP Methacrylates Cattle Adsorption medicine.drug |
| Zdroj: | Toxins Toxins; Volume 10; Issue 2; Pages: 58 Toxins, Vol 10, Iss 2, p 58 (2018) |
| ISSN: | 2072-6651 |
| Popis: | Ergot alkaloids, in their active isomeric form, affect animal health and performance, and adsorbents are used to mitigate toxicities by reducing bioavailability. Adsorbents with high specificity (molecularly imprinted polymers: MIP) adsorb ergot alkaloids in vitro, but require evaluation for biological implications. Using ex vivo myography, synthetic polymers were evaluated for effects on the bioactivity of ergotamine tartrate (ETA). Polymers were first evaluated using isotherms. Lateral saphenous veins were collected from 17 steers for four independent studies: dose response of ETA, adsorbent dose response, validation of pre-myograph incubation conditions and MIP/ non-molecularly imprinted polymer (NIP) comparison. Norepinephrine normalized percent contractile response to increasing ETA exhibited a sigmoidal dose response (max: 88.47 and log of the effective molar concentration (EC50) (−log [ETA]) of 6.66 ± 0.17 M). Although sample preparation time affected contractile response (p < 0.001), pre-myograph incubation temperature (39 vs. 21 °C, 1 h) had no effect (p > 0.05). Isothermal adsorption showed a maximum adsorption of 3.27E-008 moles·mg−1 and affinity between 0.51 and 0.57 mg (R2: 0.83–0.92) for both polymers, with no significant difference between polymers (p > 0.05). No significant differences in maximum inhibitory (p = 0.96) and IC50 responses (p = 0.163) between MIP and NIP were noticed. Normalized percent contraction could be predicted from the in vitro adsorption data (R2 = 0.87, p < 0.01), for both polymers. These studies indicate that synthetic polymers are potentially effective adsorbents to mitigate ergot toxicity caused by ergot alkaloids, with little evidence of significant differences between MIP and NIP in aqueous media. |
| Databáze: | OpenAIRE |
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