Intestinal transit of an orally administered streptomycin-rifampicin-resistant variant ofBifidobacterium longumSBT2928: its long-term survival and effect on the intestinal microflora and metabolism
| Autor: | S. Fujiwara, H. Hashiba, A. Kimura, Y. Seto |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Bifidobacterium longum medicine.drug_class Antibiotics Administration Oral Biology Applied Microbiology and Biotechnology Microbiology Clostridia Feces Clostridium Ammonia Oral administration Surveys and Questionnaires medicine Humans Ingestion Intestinal Mucosa Bacteria Probiotics Human gastrointestinal tract Genetic Variation Drug Resistance Microbial General Medicine Hydrogen-Ion Concentration biology.organism_classification Enterobacteriaceae Drug Resistance Multiple Random Amplified Polymorphic DNA Technique Intestines medicine.anatomical_structure Mutation Female Bifidobacterium Actinomycetales Infections Cell Division Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 90:43-52 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | S. FUJIWARA, Y. SETO, A. KIMURA AND H. HASHIBA. 2001. Aims: The objectives of this study are to investigate the fate of a streptomycin–rifampicin-resistant variant of Bifidobacterium longum SBT2928 (BL2928SR) and the influence of its oral administration on the composition and metabolism of the intestinal microflora. Methods and Results: Intestinal passage of BL2928SR was monitored by a combination of selection with antibiotics and identification by a randomly amplified polymorphic DNA (RAPD)–PCR method. Intestinal microflora was analysed by the method developed by Mitsuoka et al. (1965, 1974). Long-term survival of orally administered BL2928SR in the human intestine was confirmed. BL2928SR ingestion specifically lowered faecal populations of Enterobacteriaceae and clostridia, including lecithinase-positive Clostridium spp. Conclusions: BL2928SR and its parent strain, BL2928, are considered to be appropriate candidates for probiotics. Significance and Impact of the Study: It is clarified that BL2928SR has the ability for long-term survival in the human gastrointestinal tract, and alters the composition and metabolism of the intestinal microflora. |
| Databáze: | OpenAIRE |
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