High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices
| Autor: | Davi Rocha Bernardes Oliveira, Rosiane Lopes da Cunha, Mariano Michelon, Guilherme de Figueiredo Furtado, Lucimara Gaziola de la Torre |
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| Rok vydání: | 2017 |
| Předmět: |
Liposome
Materials science 010304 chemical physics Microfluidics Dispersity Nanotechnology 02 engineering and technology Surfaces and Interfaces General Medicine Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 01 natural sciences Continuous production Volumetric flow rate High-Throughput Screening Assays Colloid and Surface Chemistry 0103 physical sciences Liposomes Hydrodynamics Physical and Theoretical Chemistry 0210 nano-technology Throughput (business) Nanoscopic scale Hydrodynamic flow Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 156 |
| ISSN: | 1873-4367 |
| Popis: | The microfluidic hydrodynamic flow-focusing is a simple technique for nanoscale liposome formation that provides several advantages compared to the traditional manufacturing techniques. This work aimed to perform a systematic study of the liposome formation using planar microfluidic devices with different channel aspect-ratios, as an alternative to enhance the throughput of liposome synthesis. In general, liposomes with a low polydispersity and a precise control of the size were successfully produced from alteration of the flow rate ratio and channel aspect-ratio. The higher aspect-ratio enabled the most rapid generation of liposomes with similar diameter and significant lower polydispersity index than the obtained by other batch technique. Besides, β-carotene was successfully incorporated into liposomes with efficiency of approximately 60% and the incorporation ability was not specific to a choice of microfluidic device aspect-ratio. The results suggest that the use of microfluidic devices could be employed for liposome production with a possible advantage to minimize the degree of parallelization of processes. These results demonstrate the potential technical feasibility of microfluidic processes for future industrial applications. |
| Databáze: | OpenAIRE |
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