Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles
| Autor: | Vijaya B. Joshi, Aliasger K. Salem, Brenda R. Carrillo-Conde, Balaji Narasimhan, Sean M. Geary |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Materials science Ovalbumin CpG Oligodeoxynucleotide T-Lymphocytes medicine.medical_treatment Static Electricity Biomedical Engineering Antineoplastic Agents Pharmacology Biochemistry Article Immunoglobulin G Polyethylene Glycols Biomaterials Mice Antigen Antibody Specificity Neoplasms Polyanhydrides medicine Animals Antigens Particle Size Molecular Biology biology Immunogenicity Vaccination General Medicine Survival Analysis Tumor antigen Mice Inbred C57BL Oligodeoxyribonucleotides Tumor progression Immunology biology.protein Nanoparticles Adjuvant Biotechnology |
| Zdroj: | Acta Biomaterialia. 9:5583-5589 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2012.11.001 |
| Popis: | Delivery of vaccine antigens with an appropriate adjuvant can trigger potential immune responses against cancer leading to reduced tumor growth and improved survival. In this study, various formulations of a bioerodible amphiphilic polyanhydride copolymer based on 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) and 1,6-bis(p-carboxyphenoxy) hexane (CPH) with inherent adjuvant properties were evaluated for antigen-loading properties, immunogenicity, and antitumor activity. Mice were vaccinated with 50:50 CPTEG:CPH microparticles encapsulating a model tumor antigen, ovalbumin (OVA), in combination with the Toll-like receptor-9 agonist, CpGoligonucleotide 1826 (CpG ODN). Mice treated with OVA-encapsulated CPTEG:CPH particles elicited the highest CD8+ T cell responses on day 14 and day 20 when compared to other treatment groups. This treatment group also displayed the most delayed tumor progression and the most extended survival times. Particles encapsulating OVA and CpG ODN generated the highest anti-OVA IgG1 antibody responses in mice serum but these mice did not show significant tumor protection. These results suggest that antigen-loaded CPTEG:CPH microparticles can stimulate antigen-specific cellular responses and could therefore potentially be used to promote antitumor responses in cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect