[Molecular mechanism of radiosensitizing effect of paclitaxel]
| Autor: | Wan-Wen Weng, Jian-Mei Wan, Cheng-Jiao Hong, Zong-Zhan Wang, Wen-Xiu Ding, Min Liu, Yu-Jie Xu |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Radiosensitizer
Radiation-Sensitizing Agents Cell division Paclitaxel Apoptosis Cell Cycle Proteins Radiation Tolerance KB Cells Flow cytometry chemistry.chemical_compound medicine Humans Cyclin B2 medicine.diagnostic_test Gene Expression Profiling Cell Cycle Cell cycle Molecular biology Antineoplastic Agents Phytogenic Real-time polymerase chain reaction Oncology chemistry Cell culture Particle Accelerators Cytokinesis |
| Zdroj: | Ai zheng = Aizheng = Chinese journal of cancer. 28(8) |
| Popis: | BACKGROUND & OBJECTIVE Paclitaxel is a radiosensitizer which may stabilize microtubules, block the G2/M phase of the cell cycle and thus modulate the radioresponsiveness of tumor cells. However, its potential molecular mechanisms of radiosensitization have not been well understood yet. This study was to investigate the radiosensitizing effect of paclitaxel on human oral epithelium carcinoma (KB) cell line and to explore the molecular mechanism of radiosensitization. METHODS The survival of KB cells following the treatment with paclitaxel and/or radiation was determined by colony-forming assay. The radiosensitizing effect was evaluated by calculating the sensitizing enhancement ratio (SER) with multi-target single hit model. The cell cycle distribution was analyzed by flow cytometry. Differentially expressed genes related to paclitaxel radiosensitization were screened using human Oligo microarray. Expressions of protein regulating cytokinesis 1 (PRC1) and cyclin B2 genes were confirmed by real-time quantitative PCR. RESULTS The proliferation of KB cells was significantly inhibited by paclitaxel combined with ionizing radiation. The SERD0 and SERDq were (2.40 +/- 1.87) and (12.23 +/- 2.81) respectively, when the concentration of paclitaxel was 20 nmol/l. After the treatment with paclitaxel in combination with irradiation, the percentage of G1 phase cells decreased from (48.32 +/- 2.40)% to (15.73 +/- 7.00)% (P |
| Databáze: | OpenAIRE |
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