Immunohistochemical Methods to Diagnose Atraumatic Spleen Rupture in Feline Infectious Peritonitis of Tiger (Panthera tigris)
| Autor: | Carmen Solcan, V. Floriştean, M. Lazăr, Carmen Cretu, Cristina Horhogea |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences Materials Science (miscellaneous) Spleen rupture General Biochemistry Genetics and Molecular Biology 0403 veterinary science 03 medical and health sciences biology.animal Materials Chemistry medicine General Pharmacology Toxicology and Pharmaceutics biology Tiger business.industry Process Chemistry and Technology General Engineering 04 agricultural and veterinary sciences General Chemistry General Medicine 030108 mycology & parasitology Feline infectious peritonitis Immunohistochemistry Panthera business |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 2668-8212 0034-7752 |
| DOI: | 10.37358/rc.17.5.5610 |
| Popis: | A body of an eight-year-old male tiger, originated from a Romanian zoo was brought in for pathological diagnosis to the Faculty of Veterinary Medicine. The necropsy revealed uveitis, sero-hemorrhagic peritonitis, fatty liver and kidney, haemorrhagic-necrotic lesions on the spleen, catarrhal enteritis, serous pericarditis and pulmonary anthracosis. Amyloidosis was found in the intestine, liver, lungs and kidney and spleen, associated with necrosis and the presence of hematin blocks. Positive macrophages for feline coronavirus (FCoV) were highlighted by immunohistochemistry in liver, kidney, lungs and intestinal lamina propria. Immunofluorescence (IF) examination of pericardial and peritoneal fluid was positive for feline infectious peritonitis (FIP). Confirmation of FIP coronavirus was made by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The systemic amyloidosis and the presence of hematin in peritoneal fluid and spleen parenchyma are lesions that were not mentioned as specific for FIP until now. |
| Databáze: | OpenAIRE |
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