Flexible triphase enzyme electrode based on hydrophobic porous PVDF membrane for high-performance bioassays
| Autor: | Zhaohong Wang, Jun Zhang, Dandan Wang, Yangru Chen, Haili Wang, Xinjian Feng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Kinetics Biomedical Engineering Biophysics Enzyme electrode 02 engineering and technology Carbon nanotube Biosensing Techniques 01 natural sciences law.invention chemistry.chemical_compound Glucose Oxidase law Electrochemistry 010401 analytical chemistry Substrate (chemistry) General Medicine Hydrogen Peroxide 021001 nanoscience & nanotechnology Polyvinylidene fluoride 0104 chemical sciences Anode Membrane Glucose Chemical engineering chemistry Biological Assay Polyvinyls 0210 nano-technology Layer (electronics) Porosity Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 183 |
| ISSN: | 1873-4235 |
| Popis: | Flexible bioassays based on oxidase-catalyzed and electrocatalytic cascade reactions have been widely reported. However, the fluctuant oxygen level and high anodic potential restricts the detection accuracy. To overcome these challenges, we report here a flexible triphase enzyme electrode by assembling an oxidase enzyme layer and Pt electrocatalysts onto a carbon nanotube film/porous polyvinylidene fluoride hydrophobic substrate. Such a flexible enzyme electrode has an air-liquid-solid triphase reaction zone where oxygen level is air phase dependent (constant and sufficient high), which stabilized the oxidase kinetics and enabled the cathodic measurement of enzymatic product H2O2 with minimum interferents caused from oxygen level fluctuation and many oxidizable species in analyte solution. Furthermore, the flexible triphase enzyme electrode exhibited good mechanical stability even after being bent over 600 times and an excellent air permeability, which are crucial to wearable devices that require long-term skin contact. |
| Databáze: | OpenAIRE |
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