Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa

Autor: Iracilda Zeppone Carlos, Vanderlan da Silva Bolzani, Raquel Soares, Dulce Helena Siqueira Silva, Flávia Cristine Mascia Lopes, Ana Pirraco, Isabel Azevedo, Luis Octávio Regasini, Ana Rocha
Přispěvatelé: Universidade Estadual Paulista (Unesp), Univ Porto
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine, Vol 9, Iss 1, p 15 (2009)
Popis: Made available in DSpace on 2013-08-28T14:10:22Z (GMT). No. of bitstreams: 1 WOS000267598400001.pdf: 685962 bytes, checksum: ea605f9468c4f56803868d7155d74981 (MD5) Made available in DSpace on 2013-09-30T18:05:46Z (GMT). No. of bitstreams: 2 WOS000267598400001.pdf: 685962 bytes, checksum: ea605f9468c4f56803868d7155d74981 (MD5) WOS000267598400001.pdf.txt: 46939 bytes, checksum: 7e0d4136f5311e84f32ea9e048578ccd (MD5) Previous issue date: 2009-05-22 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:21:11Z No. of bitstreams: 2 WOS000267598400001.pdf: 685962 bytes, checksum: ea605f9468c4f56803868d7155d74981 (MD5) WOS000267598400001.pdf.txt: 46939 bytes, checksum: 7e0d4136f5311e84f32ea9e048578ccd (MD5) Made available in DSpace on 2014-05-20T14:21:11Z (GMT). No. of bitstreams: 2 WOS000267598400001.pdf: 685962 bytes, checksum: ea605f9468c4f56803868d7155d74981 (MD5) WOS000267598400001.pdf.txt: 46939 bytes, checksum: 7e0d4136f5311e84f32ea9e048578ccd (MD5) Previous issue date: 2009-05-22 FCT Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ERAB, European Advisory Board Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity.Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development. São Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, Brazil Univ Porto, Fac Med, Dept Biochem FCT U38, Oporto, Portugal São Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, Brazil São Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, Brazil São Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, Brazil CAPES: 1008/07-2 ERAB, European Advisory Board: EA0641 FAPESP: 03/02176-7
Databáze: OpenAIRE
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