919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities
| Autor: | Man-Man Chen, Danqing Pan, Pengfei Gao, Xin-Yun Tian, Jing-Wei Xing, Xiuhua Peng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Leptin
Male 0301 basic medicine medicine.medical_specialty Maternal stress PPARγ media_common.quotation_subject Actinidia Appetite Anorexia 919 syrup RM1-950 Gut flora Mice 03 medical and health sciences 0302 clinical medicine Proopiomelanocortin Pregnancy Internal medicine medicine Animals media_common Pharmacology Leptin receptor biology Plant Extracts business.industry Body Weight Postpartum Period digestive oral and skin physiology General Medicine biology.organism_classification Neuropeptide Y receptor Gastrointestinal Microbiome PPAR gamma 030104 developmental biology Endocrinology Hypothalamus 030220 oncology & carcinogenesis biology.protein Female Therapeutics. Pharmacology medicine.symptom Reactive Oxygen Species business |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 138, Iss, Pp 111455-(2021) |
| ISSN: | 0753-3322 |
| Popis: | Background Women with postpartum psychiatric disorders are prone to severe anorexia. Clinical studies have revealed the efficacy of 919 syrup, a traditional Chinese medicine mixture against postpartum illnesses, such as in regulating maternal mood and improving postpartum anorexia. Aim This study investigated the mechanisms through which 919 syrup improved anorexia induced by postpartum stress, focussing on the combined peroxisome proliferator-activated receptor gamma (PPARγ) and leptin signalling pathway, and its effects on the structure of the gut flora. Methods Mice were randomly divided into five groups—control group, immobilisation stressed (IS) group (normal saline), pioglitazone (Piog; western medicine control) group, 919 syrup low-dose (TJD; 13.5 g/kg) group, and 919 syrup high-dose (TJG; 27.0 g/kg) group. The control group was housed normally. The other groups received IS for 3 h daily for 21 days. The treatments were initiated following the first postnatal day and were administered by gastric gavage. All mice were sacrificed under anaesthesia on postnatal day 22. Blood, hypothalamus, stomach, and faecal specimens were collected. Gene and protein expression levels of components of the PPARγ–leptin signalling pathway in the serum, hypothalamus, and stomach were determined. Immunofluorescence staining for proopiomelanocortin (POMC), phosphorylated signal transducer and activator of transcription 3 (pSTAT3), and leptin was performed to observe their spatial distributions in the hypothalamus and stomach. 16s rRNA gene sequencing and bioinformatics analysis of fecal specimens were performed. Results After IS, postpartum mice showed significantly reduced appetite and body weight, accompanied by abnormalities in the structure of the gut flora. Treatment with 919 syrup (27.0 g/kg) downregulated malondialdehyde and upregulated catalase, glutathione peroxidase, and superoxide dismutase by activating PPARγ, thereby affecting the expression of leptin signalling pathway components (leptin, leptin receptor, pSTAT3, POMC, and cocaine and amphetamine-related transcript and neuropeptide Y), and modulated the gut flora in stressed mice. Conclusion 919 syrup improved appetite in mice with postnatal stress by activating PPARγ to induce crosstalk with the leptin signalling pathway, this mechanism was similar to that of PPARγ agonists. 919 syrup also improved gut flora structure, and the changes in the relative abundances of the gut flora strongly correlated with the expression levels of PPARγ and leptin pathway components. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect