Regulated tissue fluidity steers zebrafish body elongation
| Autor: | Thierry Emonet, Andrew K. Lawton, Michael W. Sneddon, Nicolas Dray, Scott A. Holley, Michael J. Stulberg, Amitabha Nandi, William Pontius |
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| Přispěvatelé: | Yale University [New Haven], Research support was provided by a National Institutes of Health (NIH) predoctoral Developmental Biology training grant [T32 HD07180-29 to A.K.L.], a NIH predoctoral Genetics training grant [T32 GM007499 to M.J.S.], the National Institute of Child Health and Human Development (NICHD) [RO1 HD045738 to S.A.H.], a Research Scholar Grant from the American Cancer Society [to S.A.H.], the Raymond and Beverly Sackler Institute for Biological, Physical and Engineering Sciences [T.E. and S.A.H.], the James McDonnell Foundation [T.E.], and by the facilities and staff of the Yale University Faculty of Arts and Sciences High Performance Computing Center. Deposited in PMC for release after 12 months., We thank Joe Wolenski for microscopy support, and Jamie Schwendinger-Schreck, Bryan Leland and Patrick McMillen for comments on the manuscript. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Embryo
Nonmammalian Time Factors Tailbud [SDV]Life Sciences [q-bio] Cell Cell Count MESH: Cadherins/genetics MESH: Embryo Nonmammalian/cytology MESH: Fibroblast Growth Factors/metabolism Animals Genetically Modified 0302 clinical medicine Cell Movement MESH: Zebrafish/metabolism MESH: Embryonic Development MESH: Animals Zebrafish Wnt Signaling Pathway Research Articles 0303 health sciences MESH: Cadherins/metabolism MESH: Fibroblast Growth Factors/genetics Wnt signaling pathway MESH: Wnt Signaling Pathway Cell Polarity Gene Expression Regulation Developmental Cadherins Cell biology Biomechanical Phenomena medicine.anatomical_structure MESH: Zebrafish/genetics MESH: Embryo Nonmammalian/metabolism MESH: Cell Polarity MESH: Tail/embryology Tail Leading edge Axial skeleton MESH: Cell Movement MESH: Biomechanical Phenomena MESH: Tail/metabolism Embryonic Development Body elongation Biology Models Biological MESH: Cell Adhesion MESH: Animals Genetically Modified 03 medical and health sciences MESH: Computer Simulation MESH: Zebrafish Proteins/genetics medicine Cell Adhesion Animals Computer Simulation Cell migration MESH: Zebrafish/embryology Progenitor cell MESH: Body Patterning MESH: Gene Expression Regulation Developmental Molecular Biology 030304 developmental biology Body Patterning MESH: Zebrafish Proteins/metabolism Cadherin MESH: Cell Count MESH: Time Factors MESH: Models Biological Zebrafish Proteins Spinal cord biology.organism_classification Fibroblast Growth Factors 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2013, 140 (3), pp.573-582. ⟨10.1242/dev.090381⟩ |
| ISSN: | 1477-9129 |
| DOI: | 10.1242/dev.090381⟩ |
| Popis: | International audience; The tailbud is the posterior leading edge of the growing vertebrate embryo and consists of motile progenitors of the axial skeleton, musculature and spinal cord. We measure the 3D cell flow field of the zebrafish tailbud and identify changes in tissue fluidity revealed by reductions in the coherence of cell motion without alteration of cell velocities. We find a directed posterior flow wherein the polarization between individual cell motion is high, reflecting ordered collective migration. At the posterior tip of the tailbud, this flow makes sharp bilateral turns facilitated by extensive cell mixing due to increased directional variability of individual cell motions. Inhibition of Wnt or Fgf signaling or cadherin 2 function reduces the coherence of the flow but has different consequences for trunk and tail extension. Modeling and additional data analyses suggest that the balance between the coherence and rate of cell flow determines whether body elongation is linear or whether congestion forms within the flow and the body axis becomes contorted. |
| Databáze: | OpenAIRE |
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