Expression and localisation of kinin receptors in colorectal polyps
| Autor: | Z. Lorenc, Antoni Stadnicki, W. Zelawski, Grażyna Nowaczyk, Grzegorz Machnik, Danuta Plewka, K. Sosada |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adenoma
Male Pathology medicine.medical_specialty Receptor Bradykinin B2 Immunology Colonic Polyps Gene Expression Colorectal adenoma Biology Receptor Bradykinin B1 Biopsy Gene expression Adenoma Villous medicine Humans Immunology and Allergy Receptor Pharmacology Hyperplasia medicine.diagnostic_test Receptors Bradykinin Middle Aged Kinin medicine.disease digestive system diseases Enterocytes Hyperplastic Polyp Immunohistochemistry Female |
| Zdroj: | International Immunopharmacology. 6:997-1002 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2006.01.016 |
| Popis: | Kinins increase vascular permeability as well as mitogenesis and proliferation, hence they have a potential to promote neoplasmatic transformation. In the present study we investigated the expression profile and localization of kinin B1 and B2 receptors in colorectal polyps. The biopsy samples from various polyps were obtained during endoscopy in tubular (n = 18), villous (n = 15) and hyperplasic polyps (n = 15). The expression of genes encoding B1 and B2 was estimated by QRT-PCR TaqMan analysis. In second series B1 and B2 receptors were visualized by immunohistochemical staining in tissue specimens from colonic polyps and adjacent normal tissue. We found the highest expression of gene encoding B1 in tubular adenomas (1891 number of copies mRNA/μg total RNA ± 312 SE) which is significantly higher as compared with controls (683 ± 197 SE, p |
| Databáze: | OpenAIRE |
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