The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera

Autor: Lucyna Holec-Gąsior, Weronika Grąźlewska, Dariusz Lautenbach, Katarzyna Dzitko, Bozena Dziadek, Bartłomiej Ferra, Justyna Gatkowska
Rok vydání: 2019
Předmět:
0301 basic medicine
Serum Proteins
Physiology
Protozoan Proteins
Antibodies
Protozoan

Biochemistry
Serology
Toxoplasma Gondii
Mice
Blood serum
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
Protozoans
Multidisciplinary
Immune System Proteins
biology
Eukaryota
Recombinant Proteins
Medicine
Antibody
Toxoplasma
Toxoplasmosis
Research Article
Science
Recombinant Fusion Proteins
030106 microbiology
Immunology
Antigens
Protozoan

Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Sensitivity and Specificity
Antibodies
03 medical and health sciences
Antigen
parasitic diseases
medicine
Parasitic Diseases
Animals
Humans
Avidity
Serologic Tests
Antigens
Immunoassays
Protozoan Infections
Organisms
Toxoplasma gondii
Biology and Life Sciences
Proteins
biology.organism_classification
medicine.disease
Fusion protein
Virology
Parasitic Protozoans
030104 developmental biology
Immunoglobulin M
Immunoglobulin G
biology.protein
Immunologic Techniques
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 6, p e0217866 (2019)
ISSN: 1932-6203
Popis: This study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were tested for their reactivity with specific IgM and IgG antibodies from sera of experimentally infected mice and humans with T. gondii infection using an enzyme-linked immunosorbent assay (ELISA). In total 192 serum samples from patients with acquired T. gondii infection and 137 sera from seronegative individuals were examined. The reactivity of chimeric antigens with antibodies generated during T. gondii invasion was measured and compared to the results obtained in assays based on whole-cell Toxoplasma antigen. Chimeric proteins proved effective in differentiation between T. gondii-infected and uninfected individuals (100% sensitivity and specificity in the IgG ELISAs) which shows their potential usefulness as a replacements for TLA in standardized commercial tests for the serodiagnosis of toxoplasmosis. In addition, the chimeric proteins were tested for use in avidity determination. Obtained results were comparable to those of the corresponding commercial assays, suggesting the utility of these proteins for avidity assessment. Furthermore, this study demonstrated that the AMA1-SAG2-GRA1-ROP1 chimeric protein has the potential to distinguish specific antibodies from serum samples of individuals with the early and chronic phase of T. gondii infection.
Databáze: OpenAIRE
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