The Phosphoenolpyruvate Phosphotransferase System Regulates Vibrio cholerae Biofilm Formation through Multiple Independent Pathways
| Autor: | Paula I. Watnick, Bradley S. Pickering, Cedric Absalon, Laetitia Houot, Sarah Chang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
macromolecular substances
Biology Microbial Communities and Interactions medicine.disease_cause Microbiology Bacterial Proteins Sigma factor Commentaries Escherichia coli medicine Phosphorylation Phosphoenolpyruvate Sugar Phosphotransferase System Vibrio cholerae Molecular Biology chemistry.chemical_classification Biofilm Gene Expression Regulation Bacterial PEP group translocation carbohydrates (lipids) Metabolic pathway Enzyme chemistry Biochemistry Biofilms Mutation bacteria Carbohydrate Metabolism Phosphoenolpyruvate carboxykinase Transcription Factors |
| Zdroj: | Journal of Bacteriology. 192:3055-3067 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00213-10 |
| Popis: | The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacterial pathogens. Components of the carbohydrate-specific PTS include the general cytoplasmic components enzyme I (EI) and histidine protein (HPr), the sugar-specific cytoplasmic components enzymes IIA (EIIA) and IIB (EIIB), and the sugar-specific membrane-associated multisubunit components enzymes IIC (EIIC) and IID (EIID). Many bacterial genomes also encode a parallel PTS pathway that includes the EI homolog EI Ntr , the HPr homolog NPr, and the EIIA homolog EIIA Ntr . This pathway is thought to be nitrogen specific because of the proximity of the genes encoding this pathway to the genes encoding the nitrogen-specific σ factor σ 54 . We previously reported that phosphorylation of HPr and FPr by EI represses Vibrio cholerae biofilm formation in minimal medium supplemented with glucose or pyruvate. Here we report two additional PTS-based biofilm regulatory pathways that are active in LB broth but not in minimal medium. These pathways involve the glucose-specific enzyme EIIA (EIIA Glc ) and two nitrogen-specific EIIA homologs, EIIA Ntr1 and EIIA Ntr2 . The presence of multiple, independent biofilm regulatory circuits in the PTS supports the hypothesis that the PTS and PTS-dependent substrates have a central role in sensing environments suitable for a surface-associated existence. |
| Databáze: | OpenAIRE |
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