Graphene nanoparticles as osteoinductive and osteoconductive platform for stem cell and bone regeneration
| Autor: | Andersen Lafont, Alexandru S. Biris, Fumiya Watanabe, David A. Gerard, Robert L. Donnell, Ramadan Abdelwahed, Silke Hecht, Karrer M. Alghazali, Hoda Elkhenany, Madhu S Dhar, David E. Anderson, Shawn E. Bourdo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold Bone Regeneration Materials science Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Mesenchymal Stem Cell Transplantation Cell morphology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Tissue engineering Osteogenesis In vivo Animals General Materials Science Bone regeneration Cells Cultured Tissue Scaffolds Goats Mesenchymal stem cell Mesenchymal Stem Cells Anatomy 021001 nanoscience & nanotechnology 030104 developmental biology chemistry Nanoparticles Molecular Medicine Agarose Graphite Stem cell 0210 nano-technology Biomedical engineering |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 13:2117-2126 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2017.05.009 |
| Popis: | The potential of graphene-based nanoparticles (GNPs) has recently gained significant attention in biomedicine, especially in tissue engineering. In this study, we investigated the osteoinductive and osteoconductive effects of low oxygen content graphene (LOG) nanoparticles on adult mesenchymal stem cells (MSCs) in vitro and in vivo. We showed that adult goat MSCs were viable in the presence of 0.1 mg/mL LOG and retained their stem cell properties. A 3D scaffold made from agarose was used to encapsulate MSCs and LOG nanoparticles. Scanning electron microscopy demonstrated the cell morphology and adherence of MSCs to LOG in the 3D form. The LOG and MSCs in the 3D scaffold were xenogenically implanted into a rat unicortical tibial bone defect. The combination of MSCs and LOG nanoparticles resulted in improved active bone formation and increased mineralization. These results strengthen the applicability of LOG nanoparticles as an adjunct treatment for bone tissue engineering. |
| Databáze: | OpenAIRE |
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