cDNA cloning of putative rat acetyl-CoA transporter and its expression pattern in brain
| Autor: | Roop Singh Bora, Shinichi Ichikawa, Yoshio Hirabayashi, Akiko Kanamori |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Gene Expression In situ hybridization Biology Complementary DNA Gene expression Genetics Animals Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Peptide sequence In Situ Hybridization Genetics (clinical) Cellular localization Messenger RNA Base Sequence Brain Gene Expression Regulation Developmental Membrane Proteins Membrane Transport Proteins Sequence Analysis DNA Blotting Northern Molecular biology Rats Olfactory bulb Anterior olfactory nucleus Carrier Proteins |
| Zdroj: | Cytogenetic and Genome Research. 89:204-208 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000015613 |
| Popis: | Rat acetyl-CoA transporter gene (Acatn) encodes a hydrophobic multi-transmembrane protein involved in the O-acetylation of gangliosides. O-acetylated gangliosides have been found to play important roles in the embryonic development of the nervous system. We have isolated rat Acatn cDNA by PCR cloning. The amino acid sequence of rat Acatn exhibited 92% and 96% homology with human and mouse sequences, respectively. The mRNA was expressed in brain at all developmental stages. Acatn expression was higher in embryonic and postnatal rats than in adult rats. Cellular localization of Acatn mRNA in adult rat brain was also analyzed by in situ hybridization. Acatn mRNA expression was detected in the neuronal cells of cerebellum, hippocampus, hypothalamus, cortex, olfactory bulb, and dorsal and ventral anterior olfactory nucleus in adult rat brain. |
| Databáze: | OpenAIRE |
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