Comparison of Two Analytical Platforms for CSF Biomarkers of Alzheimer's Disease
| Autor: | Maria Empar Blanco Cantó, C. Muñoz-Ruiz, C. Leiva-Santana, J. Sánchez-Payá, Jose Antonio Monge-Argilés, Ruth Gasparini Berenguer |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Analyte Pathology medicine.medical_specialty Article Subject Correlation coefficient lcsh:Medicine General Biochemistry Genetics and Molecular Biology Chemistry Techniques Analytical Correlation Alzheimer Disease medicine Humans Multiplex Aged Demography Chromatography General Immunology and Microbiology Receiver operating characteristic business.industry lcsh:R Area under the curve Conversion factor General Medicine ROC Curve Area Under Curve Csf biomarkers Female business Biomarkers Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Cerebrospinal fluid (CSF) biomarkers of Alzheimer’s disease (AD) are currently being assessed with two different assays. Our objective was to study if there is a correlation between values obtained by both techniques, to compare their validity and search for conversion factor between values obtained for every protein. We compared the performances of two commonly used platforms, an enzyme-linked immunosorbent assay (ELISA) and a multiplex (xMAP) technology for measurement of CSFAβ1–42, total tau (T-tau), and phosphorylated tau 181 (P-tau181p) proteins, in 30 AD patients and 28 control subjects. The relations between the variables of both techniques were evaluated using the Spearmanpcorrelation coefficient (α=0.05). Receiver operating characteristic and area under the curve (AUC) analyses were calculated for the variables of both techniques. The two assays platforms yielded different absolute values for the various analytes, always higher in ELISA. We found some correction factor between values: 2,1- to 3-fold forAβ1–42; 4,1- to 4,6-fold for T-tau; and 1,4- to 1,6-fold forP-tau181p. In addition, those values were highly correlated (Aβ1–42:r=0.70,P<0.01; T-tau:r=0.90,P<0.01;P-tau181p:r=0.85,P<0.01) and the AUC for the variables showed very similar values. In conclusion, the results obtained with ELISA and xMAP platforms were highly correlated and its validity is very similar. Differences in absolute values point to the need for a clear description of the technique used. Moreover, we found some conversion factor between values of every protein that may be useful for transformation between both techniques. |
| Databáze: | OpenAIRE |
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