The DNA replication-related element-binding factor (DREF) is a transcriptional regulator of the Drosophila myb gene
| Autor: | Gary Ramsay, Nikolai V. Sharkov, Alisa L. Katzen |
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| Rok vydání: | 2002 |
| Předmět: |
animal structures
Transcription Genetic 5' Flanking Region Genes myb TATA box Recombinant Fusion Proteins 5' flanking region Molecular Sequence Data Biology Cell Line Proto-Oncogene Proteins c-myb Genetics Transcriptional regulation Animals Drosophila Proteins MYB Luciferases Promoter Regions Genetic Transcription factor Regulation of gene expression Binding Sites fungi Downstream promoter element General Medicine Molecular biology TATA Box Drosophila melanogaster Gene Expression Regulation Protein Biosynthesis Transcription Initiation Site 5' Untranslated Regions Transcription Factors |
| Zdroj: | Gene. 297(1-2) |
| ISSN: | 0378-1119 |
| Popis: | Drosophila melanogaster possesses a single gene, Dm myb, that is closely related to the vertebrate proto-oncogene c-Myb, and its other family members (A-Myb and B-Myb), all of which encode transcription factors. Dm myb is expressed in all proliferating cells throughout development, and previous studies demonstrate that Dm myb promotes both S-phase and M-phase in proliferating cells, while preserving diploidy by suppressing endoreduplication. We have initiated a characterization of the mechanisms that regulate Dm myb expression, and we report here that the transcriptional activator DREF (the DNA replication-related element binding factor) activates Dm myb transcription via two binding sites located in the 5' flanking region; that the Dm myb promoter lacks a prototypical TATA box sequence and instead appears to use an initiator/downstream promoter element (Inr/DPE) type promoter; and that Dm myb expression is regulated at the translational as well as transcriptional level. |
| Databáze: | OpenAIRE |
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