Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism
Autor: | Karolina Antosik, Maciej Borowiec, Marlena Broncel, Emilia Luczak, Paulina Gorzelak-Pabis, Katarzyna Wojdan, Maciej Chałubiński |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Microbiology (medical) Endothelium FcεRI Cell lcsh:Medicine Immunoglobulin E Umbilical vein 03 medical and health sciences Antigen medicine Humans Receptor Cells Cultured biology Mechanism (biology) Chemistry Receptors IgE lcsh:R IL-4 Endothelial Cells FcεRII Cell biology Antibodies Anti-Idiotypic 030104 developmental biology Infectious Diseases medicine.anatomical_structure biology.protein Interleukin-4 Function (biology) |
Zdroj: | Postępy Higieny i Medycyny Doświadczalnej, Vol 71, Iss 1, Pp 162-169 (2017) |
ISSN: | 1732-2693 0032-5449 |
Popis: | Background: Human vascular endothelial function and integrity may be regulated by many non-specific factors. However, the potential influence of specific antigens via an IgE-mediated mechanism remains unknown. The aim of the study was to determine the expression of the IgE receptors FcεRI and FcεRII in the human vascular endothelium and to assess their relevance in the IgE-mediated regulation of endothelial integrity. Material/Methods: FcεRI and FcεRII expression in human umbilical vein endothelial cells (HUVEC) was genetically assessed by PCR with respective primers and sequencing. HUVEC were cultured with IL-4, and changes in FcεRI and FcεRII mRNA expression were analyzed by real-time PCR. Changes in the integrity of endothelium pre-treated with anti-BSA-DNP IgE following exposure to the specific BSA-DNP antigen was assessed using the Real-time Cell Electric Impedance Sensing system (RTCA-DP). Results: PCR and sequencing revealed the expression of FcεRI and FcεRII receptors in the human vascular endothelium. IL-4 caused respective 2- and 3-fold increases in FcεRI and FcεRII mRNA expression. Exposure of endothelium pre-treated with anti-BSA-DNP IgE to specific BSA-DNP antigen led to a 20% increase of endothelial integrity (pConclusions: The presence of FcεRI and FcεRII may allow the human vascular endothelium to respond to a specific antigen by increasing its integrity via an IgE-mediated mechanism. |
Databáze: | OpenAIRE |
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