Cytogenetic and cellular events during radiation-induced thymic lymphomagenesis in the p53 heterozygous (±) B10 mouse
| Autor: | Kimio Tanaka, Masahiko Mori, H. Tsuji, Shiro Aizawa, Kazuko Yoshida, Keiko Watanabe, Tohru Inoue, Yoko Hirabayashi, Hitoko Kamisaku |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Heterozygote
medicine.medical_specialty Neoplasms Radiation-Induced Tumor suppressor gene T-Lymphocytes Loss of Heterozygosity Biology Lymphoma T-Cell medicine.disease_cause Loss of heterozygosity Cytogenetics Mice Gene duplication Biomarkers Tumor medicine Animals Radiology Nuclear Medicine and imaging Allele Bone Marrow Transplantation Mice Knockout Membrane Glycoproteins Radiological and Ultrasound Technology Chromosome Thymus Neoplasms Genes p53 medicine.disease Lymphoma Mice Inbred C57BL Cancer research Carcinogenesis Precancerous Conditions Microsatellite Repeats |
| Zdroj: | International Journal of Radiation Biology. 78:165-172 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553000110097965 |
| Popis: | Cellular and cytogenetic events in radiation-induced thymic lymphomagenesis were investigated in the p53 heterozygous (+/-) mouse following a single dose of whole-body irradiation.The loss of the wild-type p53 allele and microsatellite markers of chromosome 11 in thymic lymphomas that developed in the p53 heterozygous (+/-) mouse after irradiation, and the stage at which prelymphoma cells appeared were analysed.The p53 heterozygous mouse developed thymic lymphomas in a dose-dependent manner. The loss of the wild-type p53 allele (loss of heterozygosity; LOH) occurred in almost all thymic lymphomas induced in the irradiated p53 heterozygous mouse. Cytogenetic analysis for the mechanism of LOH strongly suggested that the loss of the wild-type p53 gene in the lymphomas was caused by duplication of the disrupted allele through either homologous recombination or non-disjunctional chromosome duplication. The assay for prelymphoma cells suggested that a critical event in the development of prelymphoma cells occurred at least 3 weeks after irradiation.The loss of the wild-type p53 gene in thymocytes of the p53 heterozygous mouse may precede the development of prelymphoma cells after irradiation and be a valuable marker of radiation-induced leukemogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|