A review of microscopy-based evidence for the association of Propionibacterium acnes biofilms in degenerative disc disease and other diseased human tissue
| Autor: | Erin Coscia, Vincent A. Fischetti, Jonathan E. Schmitz, Andrew McDowell, Ondrej Slaby, Holger Brüggemann, Manu N. Capoor, Christof Birkenmaier, Filip Ruzicka, Søren Ohrt-Nissen, Fahad S. Ahmed, Assaf Raz, David G. Davies, Jeffrey C. Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiological culture
Disease Intervertebral Disc Degeneration Arthroscopy 0302 clinical medicine INFECTION Acne Vulgaris Medicine Orthopedics and Sports Medicine Degenerative disc disease Acne 030222 orthopedics Microscopy Prostate cancer medicine.diagnostic_test biology Biofilm SKIN PREPARATION QUANTITATIVE-ANALYSIS DIRECT VISUALIZATION BRONCHOALVEOLAR LAVAGE CELLS Intervertebral Disc Degeneration/diagnostic imaging SHOULDER Gram-Positive Bacterial Infections/diagnostic imaging BACTERIAL-DNA Immunofluorescence 03 medical and health sciences Propionibacterium acnes FISH-CLSM Humans Acne vulgaris Gram-Positive Bacterial Infections LYMPH-NODES business.industry PROSTATE TISSUE Atherosclerosis medicine.disease biology.organism_classification Acne Vulgaris/diagnostic imaging Biofilms INTERVERTEBRAL DISCS Immunology Surgery Cutibacterium acnes business 030217 neurology & neurosurgery Fluorescence in situ hybridization |
| Zdroj: | Capoor, M N, Birkenmaier, C, Wang, J C, McDowell, A, Ahmed, F S, Brüggemann, H, Coscia, E, Davies, D G, Ohrt-Nissen, S, Raz, A, Ruzicka, F, Schmitz, J E, Fischetti, V A & Slaby, O 2019, ' A review of microscopy-based evidence for the association of Propionibacterium acnes biofilms in degenerative disc disease and other diseased human tissue ', European Spine Journal . https://doi.org/10.1007/s00586-019-06086-y Capoor, M N, Birkenmaier, C, Wang, J C, McDowell, A, Ahmed, F S, Brüggemann, H, Coscia, E, Davies, D G, Ohrt-Nissen, S, Raz, A, Ruzicka, F, Schmitz, J E, Fischetti, V A & Slaby, O 2019, ' A review of microscopy-based evidence for the association of Propionibacterium acnes biofilms in degenerative disc disease and other diseased human tissue ', European Spine Journal, vol. 28, no. 12, pp. 2951-2971 . https://doi.org/10.1007/s00586-019-06086-y |
| Popis: | PURPOSE: A recent research shows an increasing recognition that organisms not traditionally considered infectious in nature contribute to disease processes. Propionibacterium acnes (P. acnes) is a gram-positive, aerotolerant anaerobe prevalent in the sebaceous gland-rich areas of the human skin. A ubiquitous slow-growing organism with the capacity to form biofilm, P. acnes, recognized for its role in acne vulgaris and medical device-related infections, is now also linked to a number of other human diseases. While bacterial culture and molecular techniques are used to investigate the involvement of P. acnes in such diseases, definitive demonstration of P. acnes infection requires a technique (or techniques) sensitive to the presence of biofilms and insensitive to the presence of potential contamination. Fortunately, there are imaging techniques meeting these criteria, in particular, fluorescence in situ hybridization and immunofluorescence coupled with confocal laser scanning microscopy, as well as immunohistochemistry.METHODS: Our literature review considers a range of microscopy-based studies that provides definitive evidence of P. acnes colonization within tissue from a number of human diseases (acne vulgaris, degenerative disc and prostate disease and atherosclerosis), some of which are currently not considered to have an infectious etiology.RESULTS/CONCLUSION: We conclude that P. acnes is an opportunistic pathogen with a likely underestimated role in the development of various human diseases associated with significant morbidity and, in some cases, mortality. As such, these findings offer the potential for new studies aimed at understanding the pathological mechanisms driving the observed disease associations, as well as novel diagnostic strategies and treatment strategies, particularly for degenerative disc disease. These slides can be retrieved under Electronic Supplementary Material. |
| Databáze: | OpenAIRE |
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