Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
| Autor: | Paula Rahal, Erica Babeto, Marilia Freitas Calmon, Fernanda Craveiro Franco, Debora Aparecida Pires de Campos Zuccari, Natalia Maria Candido, André Luis Giacometti Conceição, Jane Lopes Bonilha, José Antônio Cordeiro |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Faculdade de Medicina de São José do Rio Preto (FAMERP) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty ADAM23 Biology medicine.disease Immunohistochemistry Metastasis Giant cell tumor of bone hypermethylation Oncology CDKN2A immunohistochemistry Gene expression DNA methylation gene expression medicine Cancer research MMP14 Hypermethylation Research Paper Giant-cell tumor of bone |
| Zdroj: | PubMed Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Cancer |
| Popis: | Made available in DSpace on 2015-12-07T15:35:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2015. Added 1 bitstream(s) on 2015-12-07T15:54:19Z : No. of bitstreams: 1 PMC4466407.pdf: 930900 bytes, checksum: a477a9565f6799ac5e73f438b90bd13f (MD5) Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB. Laboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil. Center for the Study of Cancer Prognosis, FAMERP, São José do Rio Preto, Brazil. Department of Pathology, FAMERP, São José do Rio Preto, Brazil. Department of Epidemiology and Collective Health, FAMERP, São José do Rio Preto, Brazil. Laboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil. |
| Databáze: | OpenAIRE |
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