A reduction in the human adenovirus virion size through use of a shortened fibre protein does not enhance muscle transduction following systemic or localised delivery in mice
| Autor: | Lyndsay M. Murray, John A. Lunde, Rashmi Kothary, Emily McFall, Robin J. Parks, Bernard J. Jasmin |
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| Rok vydání: | 2014 |
| Předmět: |
Gene Expression Regulation
Viral Muscle tissue medicine.medical_treatment Genetic Vectors Intraperitoneal injection Biology Cell Line Viral vector Transduction Mice Viral Proteins chemistry.chemical_compound Transduction (genetics) Tibialis anterior muscle Virology medicine Adenovirus Animals Transgenes Muscle Skeletal Motor Neurons Adenoviruses Human Gene Transfer Techniques Motor neuron Molecular biology Extravasation 3. Good health medicine.anatomical_structure chemistry Polylysine Muscle Receptors Virus Capsid Proteins |
| Zdroj: | Virology. :444-453 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2014.08.026 |
| Popis: | We have investigated whether reducing the overall size of adenovirus (Ad), through use of a vector containing a shortened fibre, leads to enhanced distribution and dissemination of the vector. Intravenous or intraperitoneal injection of Ad5SlacZ (12nm fibre versus the normal Ad5 37nm fibre) or Ad5SpKlacZ (shortened fibre with polylysine motif in the H–I loop of fibre knob domain) led to similar levels of lacZ expression compared to Ad5LlacZ (native Ad5 fibre) in the liver of treated animals, but did not enhance extravasation into the tibialis anterior muscle. Direct injection of the short-fibre vectors into the tibialis anterior muscle did not result in enhanced spread of the vector through muscle tissue, and led to only sporadic transgene expression in the spinal cord, suggesting that modifying the fibre length or redirecting viral infection to a more common cell surface receptor does not enhance motor neuron uptake or retrograde transport. |
| Databáze: | OpenAIRE |
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