Isolation and characterization of the genomic region from Drosophila kuntzei containing the Adh and Adhr genes
| Autor: | Wilke Van Delden, Louis van de Zande, Jantien E. Oppentocht |
|---|---|
| Přispěvatelé: | Population Genetics, Van de Zande lab |
| Rok vydání: | 2002 |
| Předmět: |
Polymerase Chain Reaction
NUCLEOTIDE-SEQUENCE Gene Duplication codon bias FAT-BODY Genomic library Drosophila kuntzei Cloning Molecular Promoter Regions Genetic Phylogeny Genetics MELANOGASTER Structural gene Nucleic acid sequence myr SPECIES GROUP Blotting Southern Codon usage bias ALCOHOL-DEHYDROGENASE EXPRESSION Drosophila Nuclear gene Molecular Sequence Data NUCLEAR GENES Biology nucleotide diversity Evolution Molecular Species Specificity CODON USAGE Sequence Homology Nucleic Acid Animals Amino Acid Sequence RNA Messenger Enhancer Codon Molecular Biology Gene Ecology Evolution Behavior and Systematics DNA Primers Gene Library Repetitive Sequences Nucleic Acid BASE COMPOSITION Base Sequence Sequence Homology Amino Acid quinaria group Alcohol Dehydrogenase Genetic Variation DNA Blotting Northern Molecular biology MOLECULAR EVOLUTION Amino Acid Substitution |
| Zdroj: | Molecular Biology and Evolution, 19(7), 1026-1040. Oxford University Press |
| ISSN: | 0737-4038 |
| Popis: | The nucleotide sequences of the Adh and Adhr genes of Drosophila kuntzei were derived from combined overlapping sequences of clones isolated from a genomic library and from cloned PCR and inverse-PCR fragments. Only a proximal promoter was detected upstream of the Adh gene, indicating that D. kuntzei Adh is regulated by a one-promoter system. Further upstream of the Adh structural gene, an adult enhancer region (AAE) was found that contains most of the regulatory sequences described for AAEs of other Drosophila species. Analysis of the ADH protein showed an amino acid change from valine to threonine in the active site at position 189 which is also found in D. funebris but is otherwise unique among Drosophila. This difference alone may be responsible for the very low ADH activity found in this species and may cause a difference in substrate usage pattern. Codon bias in Adh and Adhr was comparable and found to be very low compared with other species. Phylogenetic analysis showed that D. kuntzei is closest related to D. funebris and D. immigrans. The time of divergence between D. kuntzei and D. funebris was estimated to be 14.2-20.2 Myr and that between D. kuntzei-D. funebris and D. immigrans to be 30.8-44.0 Myr. An analysis of the genetic variation in the Adh gene and upstream sequences Of four European strains showed that this gene was highly variable. Overall nucleotide diversity (pi) was 0.0139, which is two times higher than that in D. melanogaster. |
| Databáze: | OpenAIRE |
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