Integrin α7 expression is increased in asthmatic patients and its inhibition reduces Kras protein abundance in airway smooth muscle cells
| Autor: | Chun Ming Teoh, Chih Sheng New, Shenna Y. Langenbach, Sheryl S.L. Tan, Dorothy H.J. Cheong, Thai Tran, John Kit Chung Tam, Amanda H. Wong |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gene isoform Integrin Respiratory System lcsh:Medicine Article Extracellular matrix Proto-Oncogene Proteins p21(ras) 03 medical and health sciences 0302 clinical medicine Antigens CD Humans lcsh:Science Actin Multidisciplinary Integrin alpha Chains biology Chronic obstructive pulmonary disease lcsh:R Muscle Smooth respiratory system Phenotype Transmembrane protein Asthma 3. Good health respiratory tract diseases 030104 developmental biology Mutation Cancer research biology.protein lcsh:Q Signal transduction 030217 neurology & neurosurgery Biomarkers Signal Transduction |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Airway smooth muscle (ASM) cells exhibit plastic phenotypic behavior marked by reversible modulation and maturation between contractile and proliferative phenotypic states. Integrins are a class of transmembrane proteins that have been implicated as novel therapeutic targets for asthma treatment. We previously showed that integrin α7 is a novel marker of the contractile ASM phenotype suggesting that targeting this protein may offer new avenues to counter the increase in ASM cell mass that underlies airways hyperresponsiveness (AHR) in asthma. We now determine whether inhibition of integrin α7 expression would revert ASM cells back to a proliferative phenotype to cause an increase in ASM cell mass. This would be detrimental to asthmatic patients who already exhibit increased ASM mass in their airways. Using immunohistochemical analysis of the Melbourne Epidemiological Study of Childhood Asthma (MESCA) cohort, we show for the first time that integrin α7 expression in patients with severe asthma is increased, supporting a clinically relevant role for this protein in asthma pathophysiology. Moreover, inhibition of the laminin-integrin α7 signaling axis results in a reduction in smooth muscle-alpha actin abundance and does not revert ASM cells back to a proliferative phenotype. We determined that integrin α7-induced Kras isoform of p21 Ras acts as a point of convergence between contractile and proliferative ASM phenotypic states. Our study provides further support for targeting integrin α7 for the development of novel anti-asthma therapies. |
| Databáze: | OpenAIRE |
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