Visualization of collagenase-sensitive acetylcholinesterase in isolated cardiomyocytes and in heart tissue
| Autor: | Mahboubeh Eghbali, Sam Seifter, Thomas F. Robinson, I. Silman |
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| Rok vydání: | 1988 |
| Předmět: |
Male
medicine.medical_specialty Histology Cell Separation In Vitro Techniques Pathology and Forensic Medicine chemistry.chemical_compound Antibody Specificity Laminin Internal medicine medicine Animals Myocyte Basement membrane biology Myocardium Skeletal muscle Heart Cell Biology Immunohistochemistry Acetylcholinesterase Molecular biology Rats Inbred F344 Rats Microbial Collagenase Endocrinology medicine.anatomical_structure chemistry Microbial collagenase biology.protein Collagenase Basal lamina medicine.drug |
| Zdroj: | Cell And Tissue Research. 253 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00222282 |
| Popis: | Previous studies have indicated that the asymmetric form of acetylcholinesterase (collagen-tailed) is localized in the basal lamina of the neuromuscular junction of skeletal muscle. The present study shows localization of the asymmetric acetylcholinesterase in the heart of the rat. Antiserum to 14 + 18 S acetylcholinesterase of the electric eel was raised in rabbits. The purified antibody did not react with collagen type I or laminin. Collagenase reduced the immunoreactivity of the enzyme with the purified antibody. Isolated cardiomyocytes and frozen sections of the heart were stained for acetylcholinesterase with the antibody. Diffuse immunofluorescence appeared over the surface of the cardiomyocytes. In the frozen sections, the immunofluorescence was most intense at the cell boundaries. These data suggest that collagenase-sensitive acetylcholinesterase in the heart is present in the myocytes and occurs in the vicinity of the basal lamina. |
| Databáze: | OpenAIRE |
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