EFFECT OF HEMORRHAGIC SHOCK ON ENDOTOXIN-INDUCING TNF PRODUCTION AND INTRA-TISSUE LIPOPOLYSACCHARIDE-BINDING PROTEIN mRNA EXPRESSION AND THEIR RELATIONSHIP
| Autor: | Jianxin Jiang, Pei-fang Zhu, You-fang Diao, Kun-lun Tian, Hui-sun Chen, Zhengguo Wang |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Resuscitation Lipopolysaccharide Kupffer Cells medicine.medical_treatment Population Gene Expression Shock Hemorrhagic Kidney Critical Care and Intensive Care Medicine chemistry.chemical_compound In vivo Internal medicine Leukocytes medicine Animals RNA Messenger education Lung education.field_of_study Membrane Glycoproteins biology Tumor Necrosis Factor-alpha Chemistry Rats Endotoxins Endocrinology Cytokine Liver Shock (circulatory) Emergency Medicine biology.protein Tumor necrosis factor alpha medicine.symptom Carrier Proteins Lipopolysaccharide binding protein Acute-Phase Proteins |
| Zdroj: | Shock. 7:206-210 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/00024382-199703000-00009 |
| Popis: | In order to further elucidate effect of hemorrhagic shock on endotoxin-inducing cytokine production, the present study was designed to investigate the production of tumor necrosis factor alpha (TNF alpha) induced by low-dose (1 microgram/kg) of lipopolysaccharide (LPS) and its cellular sources after hemorrhagic shock (HS) in rats. With combination of expression of lipopolysaccharide-binding protein (LBP) mRNA in the liver, lungs, and kidneys, we further analyzed a possible mechanism for increasing sensitivity to LPS by shock. We found in vivo that plasma TNF alpha levels in the HS + LPS group were 20-fold higher than those in the HS group (p < .01) and 2.7-fold higher than those in the LPS group (p < .05). It was shown in vitro that the capacity of the peripheral white blood cells to produce TNF alpha in response to LPS stimulation was significantly decreased by 126% (p < .01) and 57% (p < .05) compared with the pre-shock levels and sham group, respectively, at the end of resuscitation following shock, and still markedly inhibited 3 h after resuscitation, while the capacity of hepatic Kupffer's cells to produce TNF alpha was significantly increased by 110% compared with the sham group (p < .01) after shock and resuscitation. Results from RT-PCR showed that expression of LBP mRNA in the liver, lungs, and kidneys was increased after shock and resuscitation. It is suggested that hemorrhagic shock could significantly strengthen endotoxin to induce TNF alpha production, which might be due to up-regulation of LBP expression in tissues after shock, and the tissue macrophage population may be the main source for cytokine production in shock. |
| Databáze: | OpenAIRE |
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