Spectroscopic investigations of Panulirus interruptus hemocyanin in the crystalline state
| Autor: | Wim G. J. Hol, B. R. Dobson, Kor H. Kalk, Martin C. Feiters, M. G. Vincent, Elisabeth Bouwman, Anne Volbeda, Jan Reedijk |
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| Rok vydání: | 1989 |
| Předmět: |
Absorption spectroscopy
medicine.medical_treatment chemistry.chemical_element Biochemistry law.invention Ultraviolet visible spectroscopy law medicine Animals Molecule Crystallization Electron paramagnetic resonance Chemistry Spectrum Analysis X-Rays Electron Spin Resonance Spectroscopy Hemocyanin Copper Nephropidae Crystallography Hemocyanins Spectrophotometry Ultraviolet Oxidation-Reduction Monoclinic crystal system |
| Zdroj: | European Journal of Biochemistry. 181:669-673 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1989.tb14775.x |
| Popis: | Single-crystal ultraviolet spectroscopy, X-ray absorption spectroscopy and EPR measurements have been used to examine the oxidation and oxygenation state of the dinuclear copper site of several types of hemocyanin crystals. The crystals contain Panulirus interruptus hemocyanin which forms hexameric molecules with a molecular mass of approximately 470 kDa. Three types of crystals have been investigated. Type-I monoclinic crystals, which have been used for the X-ray structure determination, contain virtually only deoxyhemocyanin. Type-II monoclinic crystals, which are less well ordered than the type-I crystals, contain a mixture of deoxy, oxy and met forms. Older crystals contain relatively more methemocyanin. A third, hexagonal, crystal form is also partially oxygenated, and, like the type-II monoclinic form, subject to gradual conversion to methemocyanin. |
| Databáze: | OpenAIRE |
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