Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly
| Autor: | Sara Sandin, Tra Huong Nguyen, Laxmi Iyer Ravi, Alexander Ludwig, Daniel Leong, Boon Huan Tan, Richard J. Sugrue |
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| Přispěvatelé: | School of Biological Sciences |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Caveolin viruses Caveolin 1 Respiratory syncytial virus Biology Caveolae Models Biological Virus Viral Proteins 03 medical and health sciences PTRF Viral envelope Morphogenesis Humans Virus envelope Lipid raft Actin Protein Stability Virus Assembly Cell Membrane RNA-Binding Proteins virus diseases Cell Biology Raft respiratory system Actins Cell biology 030104 developmental biology Respiratory Syncytial Virus Human Cavin HeLa Cells Protein Binding Research Article |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.198853 |
| Popis: | Respiratory syncytial virus (RSV) is an enveloped virus that assembles into filamentous virus particles on the surface of infected cells. Morphogenesis of RSV is dependent upon cholesterol-rich (lipid raft) membrane microdomains, but the specific role of individual raft molecules in RSV assembly is not well defined. Here, we show that RSV morphogenesis occurs within caveolar membranes and that both caveolin-1 and cavin-1 (also known as PTRF), the two major structural and functional components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G protein. Moreover, cavin-1 protein levels were significantly increased in RSV-infected cells, leading to a virus-induced change in the stoichiometry and biophysical properties of the caveolar coat complex. Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus contributes to defining the biological properties of the RSV envelope. Highlighted Article: The assembly of respiratory syncytial virus occurs in caveolar membranes and caveolar coat components are incorporated into the viral envelope. |
| Databáze: | OpenAIRE |
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