Experimental Reptarenavirus Infection of Boa constrictor and Python regius
| Autor: | Theresa Pesch, Udo Hetzel, Jussi Hepojoki, Anja Kipar, Leonora Szirovicza, Olli Vapalahti, Yegor Korzyukov, Saskia Keller |
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| Přispěvatelé: | University of Zurich, Pfeiffer, Julie K, Hepojoki, Jussi |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
1109 Insect Science
040301 veterinary sciences Cytoplasmic inclusion medicine.medical_treatment Immunology Intraperitoneal injection 10184 Institute of Veterinary Pathology Microbiology Virus 0403 veterinary science Pathogenesis 03 medical and health sciences Immune system Virology medicine Hantavirus 030304 developmental biology 2403 Immunology 0303 health sciences biology 2404 Microbiology 04 agricultural and veterinary sciences biology.organism_classification 3. Good health Nucleoprotein Vaccination Titer Insect Science 2406 Virology 570 Life sciences Pathogenesis and Immunity Boa constrictor |
| Zdroj: | J Virol |
| Popis: | Boid inclusion body disease (BIBD) causes losses in captive constrictor snake populations globally. BIBD associates with formation of cytoplasmic inclusion bodies (IB) which mainly comprise reptarenavirus nucleoprotein (NP). In 2017, BIBD was reproduced by cardiac injection of boas and pythons with reptarenaviruses, thus demonstrating a causative link between reptarenavirus infection and the disease. Herein, we report experimental infections of pythons (N=16) and boas (N=16) with three reptarenavirus isolates. First, we used pythons (N=8) to test two virus delivery routes: intraperitoneal injection and tracheal instillation. Independent of the delivery route, we detected viral RNA but no IBs in tissues two weeks post inoculation. Next, we inoculated pythons (N=8) via the trachea. During the four month following the infection snakes showed transient central nervous system (CNS) signs but lacked detectable IB at the time of euthanasia. One of the snakes developed severe CNS signs and we succeeded in re-isolating the virus from the brain of this individual, and could demonstrate viral antigen in neurons. In a third attempt, we tested co-housing, vaccination, and sequential infection with multiple reptarenavirus isolates on boas (N=16). At 10 months post inoculation all except one snake tested positive for viral RNA but none exhibited the characteristic IB. Analysis of the antibody responses demonstrated lower neutralizing but higher anti-reptarenavirus NP titers in experimentally versus naturally reptarenavirus infected boas. Our findings suggest that in addition to reptarenavirus infection, other factors, e.g. the antibody response, contribute to BIBD pathogenesis.IMPORTANCEA 2017 study demonstrated cardiac reptarenavirus injection to induce boid inclusion body disease (BIBD) in pythons and boas. In the present study, we experimentally infected pythons and boas with reptarenavirus via either intraperitoneal injection or tracheal instillation. We found both virus delivery routes to result in infection; though the latter could reflect the natural route of infection. In the experimentally infected snakes, we did not find evidence of inclusion body (IB) formation, characteristic to BIBD, in pythons or in boas. Most of the snakes (11/12) studied were reptarenavirus infected after ten-month follow up, which suggests that they could eventually have developed BIBD. We further found differences between the antibody responses of experimentally and naturally reptarenavirus infected snakes, which could indicate that the pathogenesis of BIBD involves factors additional to reptarenavirus infection. As snakes are poikilotherm, also the housing conditions could have an effect. |
| Databáze: | OpenAIRE |
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