The power of line-narrowing techniques: Applications to photosynthetic chromoprotein complexes and autofluorescent proteins
| Autor: | S. Jezowski, Silvia Völker, S. Bonsma, F. Konz, J. Gallus, R. Purchase |
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| Přispěvatelé: | Physics and Astronomy, Biophysics Photosynthesis/Energy |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Photosynthetic reaction centre
biology Photosystem II Chemistry biology.organism_classification Photochemistry Photosynthesis Purple bacteria Fluorescence Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Chromoprotein Astrophysics::Solar and Stellar Astrophysics SDG 7 - Affordable and Clean Energy Spectroscopy Absorption (electromagnetic radiation) |
| Zdroj: | Purchase, R, Bonsma, S, Jezowski, S, Gallus, J, Konz, F & Volker, S L 2005, ' The power of line-narrowing techniques: Applications to photosynthetic chromoprotein complexes and autofluorescent proteins ', Optics and Spectroscopy, vol. 98, no. 5, pp. 699-711 . https://doi.org/10.1134/1.1929056 Optics and Spectroscopy, 98(5), 699-711. Springer Science + Business Media |
| ISSN: | 0030-400X |
| Popis: | Laser spectroscopic techniques at low temperature, such as fluorescence line-narrowing and hole burning, enable an increase of spectral resolution by a factor of 103–105 compared to conventional spectroscopy at room temperature. With these methods, it is possible to retrieve a fingerprint of the species involved and to measure the rates of dynamic processes that normally remain hidden in the broad absorption bands. A few applications carried out in our laboratory will be discussed: (1) the determination of energy transfer rates in the peripheral LH2 complex of purple bacteria; (2) the study of spectral diffusion and its implications in three types of systems: (a) the B820 and B777 subunits of the LH1 complex of purple bacteria, (b) the photosystem II reaction center (PSII RC) and CP47 antenna complex of green plants, and (c) an organic glass doped with bacteriochlorophyll a; (3) the unraveling of 0-0 transitions and the pathways of photoconversion between a number of conformations of the green fluorescent protein mutant S65T; (4) the measuring of electron-phonon coupling strengths in PSII RC and the red fluorescent protein DsRed; and (5) the determination and comparison of the homogeneous linewidths and optical dephasing in photosynthetic chromoprotein complexes and autofluorescent proteins. |
| Databáze: | OpenAIRE |
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