Mechanical environment, donor age, and presence of endothelium interact to modulate porcine artery viability ex vivo
| Autor: | J. O'Brien, J. W. Gaynor, P. M. Kirshbom, Keith J. Gooch, Rebecca J. Gusic, Richard J. Myung, Valerie Clerin |
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| Rok vydání: | 2002 |
| Předmět: |
Programmed cell death
Endothelium Carotid arteries Sus scrofa Biomedical Engineering Biology Organ culture Andrology Organ Culture Techniques Tissue engineering medicine Animals Tissue Engineering Age Factors Hemodynamics Models Cardiovascular Histology Anatomy Organ Preservation Biomechanical Phenomena Perfusion medicine.anatomical_structure Carotid Arteries Animals Newborn Endothelium Vascular Ex vivo Artery |
| Zdroj: | Annals of biomedical engineering. 30(9) |
| ISSN: | 0090-6964 |
| Popis: | Though ex vivo culture of arteries is a widely used model of native arteries and is closely aligned with efforts to generate tissue-engineered arteries, the effects of culture conditions on artery viability are poorly characterized. To investigate factors regulating long-term viability of cultured arteries, carotid arteries from neonatal and adolescent pigs were perfused for up to 27 days with steady laminar flow ranging from ∼2% to ∼200% of physiological flow rates. Arteries from neonatal animals (2 weeks old, ∼5 kg) were susceptible to spontaneous progressive endothelial denudation followed by deterioration of the vessel wall that spread from luminal to abluminal regions. Subphysiological levels of flow and pressure abrogated this deterioration. Arteries harvested from adolescent (6 months old, ∼100 kg) animals maintained viability and retained structure for at least 9 days as assessed by normal histology, presence of intact endothelium, normal mitochondrial activity, and low levels of cell death and proliferation, unless the vessels were subjected to superphysiological levels of flow or the endothelium was intentionally denuded. Adolescent arteries perfused at subphysiological, but not physiological, flow rates maintained viability and normal structure for at least 27 days. These data indicate that under the appropriate conditions, arteries may be cultured long term but careful attention to the viability is merited. © 2002 Biomedical Engineering Society. PAC2002: 8780Rb, 8719Tt, 8719Uv, 8768+z |
| Databáze: | OpenAIRE |
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