Mycobacterium bovis BCG-infected neutrophils and dendritic cells cooperate to induce specific T cell responses in humans and mice
| Autor: | Macarena Robledo, Sarah Boudaly, Nathalie Winter, Céline Morel, Jean Claude Gluckman, Valérie Abadie, Brigitte Gicquel, Niclas Setterblad, Edgar Badell |
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| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS), Génétique mycobactérienne - Mycobacterial genetics, Institut Pasteur [Paris], Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7), Agence Nationale de Recherche sur le SIDA, Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2008 |
| Předmět: |
Male
Mycobacterium bovis BCG medicine.medical_treatment T cell cell cooperation BACILLUS-CALMETTE-GUERIN Immunology Mice Transgenic Cell Communication INNATE DRAINING LYMPH-NODES IMMUNITY Biology INTRACELLULAR PATHOGENS DC-SIGN TUBERCULOSIS INFECTION Microbiology Mice Immune system neutrophils Antigen T-Lymphocyte Subsets medicine Animals Humans Immunology and Allergy Cytotoxic T cell dendritic cells Cells Cultured IN-VIVO GENE-EXPRESSION Mice Knockout Hybridomas Cross-presentation Interleukin Dendritic cell Mycobacterium bovis Coculture Techniques Mice Inbred C57BL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Cytokine medicine.anatomical_structure Cytokines IL-2 PRODUCTION cross -presentation |
| Zdroj: | European Journal of Immunology European Journal of Immunology, Wiley-VCH Verlag, 2008, 38 (2), pp.437-447. ⟨10.1002/eji.2007379051⟩ European Journal of Immunology, 2008, 38 (2), pp.437-447. ⟨10.1002/eji.2007379051⟩ |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | International audience; Neutrophils are increasingly thought to modulate dendritic cell (DC) functions. We investigated the role of the neutrophil-DC partnership in the response to Mycobacterium bovis BCG-the vaccine used against tuberculosis. We compared neutrophil-DC crosstalk in humans and mice, searching for functional differences. In both species, neutrophils captured fluorescent BCG-enhanced green fluorescent protein (EGFP) and were more phagocytic than DC. Non-apoptotic BCG-infected neutrophils clustered with immature DC, establishing intimate contacts with dendrites, at which fluorescent intact bacilli were observed. Physical interactions between neutrophils and DC were required for DC activation. Human BCG-infected DC produced interleukin (IL)-10, an inhibitory cytokine, whereas DC exposed to BCG-infected neutrophils produced low to undetectable amounts of the cytokine. Mouse BCG-infected neutrophils induced sustained IL-2 production by DC. Human DC exposed to BCG-infected neutrophils stimulated recall T cell reactivity from vaccinated donors. Mouse DC infected with recombinant ovalburnin (OVA)-producing BCG (rBCG(ova)) elicited proliferation of TCR-OVA-transgenic CD4 and CD8 T cells. Moreover, exposing DC to rBCG(ova)-infected neutrophils enhanced OVA presentation. Thus, in mice and humans, neutrophils help DC to cross-present BCG antigens to T cells. Our results suggest that this "menage a trois" involving neutrophils, DC and T cells plays a major role in the immune response to BCG. |
| Databáze: | OpenAIRE |
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