Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand

Autor:	Stefan Kittler, Julian Ebner, Mihail Besleaga, Johan Larsbrink, Barbara Darnhofer, Ruth Birner-Gruenberger, Silvia Schobesberger, Christopher K. Akhgar, Andreas Schwaighofer, Bernhard Lendl, Oliver Spadiut
Rok vydání:	2022
Předmět:	Bacterial Proteins Escherichia coli Immunoglobulin Light Chains Bioengineering General Medicine Ligands Immunoglobulin Fragments Applied Microbiology and Biotechnology Chromatography Affinity Recombinant Proteins Protein Binding Biotechnology
Zdroj:	Journal of Biotechnology. 359:108-115
ISSN:	0168-1656
Popis:	Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a984197bd93c8e9e69ff8a03f080a570 https://doi.org/10.1016/j.jbiotec.2022.10.002 Zobrazit plný text záznamu Full Text from ScienceDirect