Characterization and Analysis of Anthocyanin-Related Genes in Wild-Type Blueberry and the Pink-Fruited Mutant Cultivar ‘Pink Lemonade’: New Insights into Anthocyanin Biosynthesis
| Autor: | Elizabeth L. Ogden, Mark K. Ehlenfeldt, Lisa J. Rowland, Jose V. Die, Richard W. Jones |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Anthocyanin Flavonoid Mutant Biology 01 natural sciences Blueberry anthocyanin lcsh:Agriculture Anthocyanins 03 medical and health sciences chemistry.chemical_compound flavonoid Gene 030304 developmental biology Regulator gene blueberry MYB transcription factor chemistry.chemical_classification Genetics 0303 health sciences Structural gene fungi Wild type lcsh:S food and beverages fruit biology.organism_classification qPCR chemistry Fruit Agronomy and Crop Science 010606 plant biology & botany Vaccinium |
| Zdroj: | Agronomy 10(9), 1296 (2020) Helvia. Repositorio Institucional de la Universidad de Córdoba instname Agronomy, Vol 10, Iss 1296, p 1296 (2020) Agronomy Volume 10 Issue 9 Helvia: Repositorio Institucional de la Universidad de Córdoba Universidad de Córdoba |
| Popis: | Blueberries are one of the richest sources of antioxidants, such as anthocyanins, among fruits and vegetables. Anthocyanin mutants, like the pink-fruited cultivar &lsquo Pink Lemonade&rsquo are valuable resources for investigating anthocyanin biosynthesis in blueberries. In this study, we examined expression of flavonoid pathway genes during fruit development in wild-type, blue-fruited blueberries using quantitative real-time PCR. Expression was also compared between wild-type and the pink-fruited &lsquo This revealed significantly lower expression in &lsquo than in wild-type of nearly all the structural genes examined suggesting that a transcriptional regulator of the pathway was affected. Hence, we compared expression of three known regulatory genes and found that the gene encoding the transcription factor MYB1 was expressed at a significantly lower level in &lsquo than in the wild-type. To validate the capacity of this MYB1 to regulate the transcription of anthocyanin genes in blueberries, a transient expression assay was conducted. Results indicated MYB1 overexpression enhanced anthocyanin production. Comparative sequence analysis between wild-type and mutant MYB1 variants found differences in highly conserved features suggesting a mechanistic explanation for the mutant phenotype. Collectively, the results presented here contribute to a better understanding of mechanisms regulating anthocyanin biosynthesis in Vaccinium. |
| Databáze: | OpenAIRE |
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